Physicochemical and biological study of selected hydrophobic polyethylenimine-based polycationic liposomes and their complexes with DNA. (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Physicochemical and biological study of selected hydrophobic polyethylenimine-based polycationic liposomes and their complexes with DNA. (Articolo in rivista) (literal)

Anno

• 2007-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.bmc.2006.10.066 (literal)

Alternative label

• Masotti A.1, Moretti F.2, Mancini F.3, Russo G.4, Di Lauro N.5, Checchia P.6, Marianecci C.7, Carafa M.8, Santucci E.9, Ortaggi G.10. (2007)
  Physicochemical and biological study of selected hydrophobic polyethylenimine-based polycationic liposomes and their complexes with DNA.
  in Bioorganic & medicinal chemistry (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Masotti A.1, Moretti F.2, Mancini F.3, Russo G.4, Di Lauro N.5, Checchia P.6, Marianecci C.7, Carafa M.8, Santucci E.9, Ortaggi G.10. (literal)

Pagina inizio

• 1504 (literal)

Pagina fine

• 1515 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni

• Impact Factor = 2.624 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 15 (literal)

Rivista

• Bioorganic & medicinal chemistry (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 3 (literal)

Note

• ISI Web of Science (WOS) (literal)
• PubMe (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1,4,5,6,10 = Chemistry Department, University of Study of Rome “La Sapienza,” P.le Aldo Moro 5, 00185 Rome, Italy; 2,3 = Laboratory of Molecular Oncogenesis, Regina Elena Cancer Institute, Via delle Messi D’Oro 156, 00158 Rome, Italy; 2 = Institute of Neurobiology and Molecular Medicine, National Council of Research, Viale Marx 15, 00156 Rome, Italy; 7,9 = Department of Studi di Chimica e Tecnologia delle Sostanze Biologicamente Attive, Faculty of Pharmacy, University “La Sapienza,” Rome, Italy; 8 = Department of Scienze del Farmaco, Faculty of Pharmacy, University “G. D’Annunzio,” Chieti, Italy. (literal)

Titolo

• Physicochemical and biological study of selected hydrophobic polyethylenimine-based polycationic liposomes and their complexes with DNA. (literal)

Abstract

• Non-viral gene therapy is based on the development of efficient and safe gene carrier systems able to transfer DNA into cells. Polyethylenimine (PEI), the most promising non-viral vector, with its high cationic-charge-density potential is able (1) to compact DNA in complexes (polyplexes) smaller than those formed by liposomes (lipoplexes) and (2) to destabilize the endosomal membrane by a 'proton sponge' effect. Several PEI's hydrophobic modifications were reported in the last several years but in some cases a reduced transfection efficiency was observed. The mechanism underlying this phenomenon is not well understood so far. In order to extensively investigate these mechanisms, we reported a physicochemical and biological study of selected hydrophobic PEI's derivatives grafted with chains of different length and percentages of substitution able to form vesicles (polycationic liposomes) and to bind DNA. Their properties were studied by means of dynamic light scattering, freeze-fracture microscopy, potentiometric titrations, gel retardation assays, polyanion exchange reactions, toxicity assays, in vitro transfections, and fluorescence microscopy. Our results indicate that even if polyplexes are able to pass through the cellular membrane, the stability of PEI's hydrophobic polyplexes likely explain their different transfection efficiency in vitro. (literal)

Prodotto di

• Sviluppo, Differenziamento e Trasformazione Cellulare (SV.P15.006.001) (Modulo)
• Istituto di neurobiologia e medicina molecolare (INMM) (Istituto)

Autore CNR

• FABIOLA MORETTI (Unità di personale interno)

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• Parole chiave di "Physicochemical and biological study of selected hydrophobic polyethylenimine-based polycationic liposomes and their complexes with DNA." (Insieme di parole chiave)

Incoming links:

Autore CNR di

• FABIOLA MORETTI (Unità di personale interno)

Prodotto

• Istituto di neurobiologia e medicina molecolare (INMM) (Istituto)
• Sviluppo, Differenziamento e Trasformazione Cellulare (SV.P15.006.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Bioorganic & medicinal chemistry (Rivista)

Insieme di parole chiave di

• Parole chiave di "Physicochemical and biological study of selected hydrophobic polyethylenimine-based polycationic liposomes and their complexes with DNA." (Insieme di parole chiave)