The analysis of serum effects on structure, size and toxicity of DDAB-DOPE and DC-Chol-DOPE lipoplexes contributes to explain their different transfection efficiency. (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• The analysis of serum effects on structure, size and toxicity of DDAB-DOPE and DC-Chol-DOPE lipoplexes contributes to explain their different transfection efficiency. (Articolo in rivista) (literal)

Anno

• 2006-01-01T00:00:00+01:00 (literal)

Alternative label

• Esposito C.a, Generosi J., Mossa G.b, Masotti A., Castellano A.C. (2006)
  The analysis of serum effects on structure, size and toxicity of DDAB-DOPE and DC-Chol-DOPE lipoplexes contributes to explain their different transfection efficiency.
  
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Esposito C.a, Generosi J., Mossa G.b, Masotti A., Castellano A.C. (literal)

Pagina inizio

• 187 (literal)

Pagina fine

• 192 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni

• Impact Factor = 1.789 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 53 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• a,b = Istituto di Neurobiologia e Medicina Molecolare-CNR, Via DEL Fosso del Cavaliere 100, 00133 Roma, Italy. (literal)

Titolo

• The analysis of serum effects on structure, size and toxicity of DDAB-DOPE and DC-Chol-DOPE lipoplexes contributes to explain their different transfection efficiency. (literal)

Abstract

• The effect of serum on structural properties of dimethyl-dioctadecyl-ammonium bromide (DDAB)-1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) liposomes and DDAB-DOPE/DNA lipoplexes has been investigated by energy dispersive X-ray diffraction (EDXD) technique, at different cationic lipid/DNA weight ratios (rho). The role of serum on the size of lipoplexes has also been studied by dynamic light scattering. Lipoplex transfection efficiency (TE) as a function of rho, and lipoplex toxicity to C6 rat glioma cells have been evaluated in Dulbecco's Modified Eagle Medium (DMEM) with and without serum. A multi-parametric analysis concerning the role of size, structure and cytotoxicity on transfection efficiency contributes to explain the experimental observation that 3beta-[N-(N',N'-dimethylaminoethane)carbamoyl]-cholesterol (DC-Chol)-DOPE/DNA transfect C6 cells better than DDAB-DOPE/DNA lipoplexes. (literal)

Prodotto di

• Istituto di neurobiologia e medicina molecolare (INMM) (Istituto)

Autore CNR

• CLAUDIO ESPOSITO (Unità di personale interno)
• GIUSEPPE MOSSA (Unità di personale interno)

Insieme di parole chiave

• Keywords of "The analysis of serum effects on structure, size and toxicity of DDAB-DOPE and DC-Chol-DOPE lipoplexes contributes to explain their different transfection efficiency." (Insieme di parole chiave)

Incoming links:

Autore CNR di

• CLAUDIO ESPOSITO (Unità di personale interno)
• GIUSEPPE MOSSA (Unità di personale interno)

Prodotto

• Istituto di neurobiologia e medicina molecolare (INMM) (Istituto)
• http://www.cnr.it/ontology/cnr/individuo/modulo/ID2348

Insieme di parole chiave di

• Keywords of "The analysis of serum effects on structure, size and toxicity of DDAB-DOPE and DC-Chol-DOPE lipoplexes contributes to explain their different transfection efficiency." (Insieme di parole chiave)