http://www.cnr.it/ontology/cnr/individuo/prodotto/ID4683
Influence of local environment on the differentiation of neural stem cells engrafted onto the injured spinal cord (Articolo in rivista)
- Type
- Label
- Influence of local environment on the differentiation of neural stem cells engrafted onto the injured spinal cord (Articolo in rivista) (literal)
- Anno
- 2006-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1179/016164106X115134 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Ricci-Vitiani L.1; Casalbore P.2; Petrucci G.3; Lauretti L.4; Montano N.4; Larocca L.M.3; Falchetti M.L.2; Lombardi D.G.1; Di Giorgio Gerevini V.5; Cenciarelli C.2; DAlessandris Q.G.4; Fernandez E.4; De Maria R.1; Maira G.4; Peschle C.1; Parati E. 6; Pallini R.4 (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni
- Impact Factor = 1.573 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- 1 = Department of Hematology, Oncology and Molecular Medicine, Istituto Superiore di Sanità, Rome, Italy;
2 = Institute of Neurobiology and Molecular Medicine, CNR, Rome, Italy;
3 = Institute of Human Pathology, Catholic University School of Medicine, Rome, Italy;
4 = Department of Neurosurgery, Catholic University School of Medicine, Rome, Italy;
5 = Department of Human Physiology and Pharmacology, 'La Sapienza' University, Rome, Italy;
6 = Laboratory of Neurobiology, Department of Neurobiology and Neurorestorative Therapies, National Neurological Institute C. Besta, Milan, Italy. (literal)
- Titolo
- Influence of local environment on the differentiation of neural stem cells engrafted onto the injured spinal cord (literal)
- Abstract
- OBJECTIVES: In vitro, neural stem cells (NSCs) proliferate as undifferentiated spheroids and differentiate into neurons, astrocytes and oligodendrocytes. These features make NSCs suitable for spinal cord (SC) reconstruction. However, in vivo experiments have demonstrated that in the injured SC transplanted NSCs either remain undifferentiated or differentiate into the astrocytic phenotype. The microenvironment of the injured SC is believed to play a crucial role in driving the differentiation of the engrafted NSCs. Here, we tested the hypothesis that inflammatory cytokines (ICs) may be involved in the restricted differentiation of NSCs after grafting onto the injured SC. METHODS: As the first step, we used immunohistochemistry to analyse the expression of tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta and interferon (IFN)-gamma in the normal SC of mice and following traumatic injury. Then, we investigated whether a combination of TNF-alpha, IL-1beta and IFN-gamma may affect the phenotype of murine NSCs in vitro. RESULTS: We found that TNF-alpha, IL-1beta and IFN-gamma, which are absent in the normal SC, are all expressed in the injured SC and the expression of these cytokines follows a timely tuned fashion with IFN-gamma being detectable as long as 4 weeks after injury. In culture, exposure of proliferating NSCs to a combination of TNF-alpha, IL-1beta and IFN-gamma was per se sufficient to induce the astrocytic differentiation of these cells even in the absence of serum. CONCLUSIONS: In the traumatically injured SC, differentiation of engrafted NSCs is restricted towards the astrocytic lineage because of the inflammatory environment. ICs are likely to play a major role in differentiation of NSCs in the in vivo conditions. (literal)
- Prodotto di
- Autore CNR
- Insieme di parole chiave
Incoming links:
- Autore CNR di
- Prodotto
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi
- Insieme di parole chiave di
