Low density lipoprotein aged in plasma forms clusters resembling subendothelial droplets: aggregation via surface sites. (Articolo in rivista)

Type
Label
  • Low density lipoprotein aged in plasma forms clusters resembling subendothelial droplets: aggregation via surface sites. (Articolo in rivista) (literal)
Anno
  • 2006-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1529/biophysj.105.075788 (literal)
Alternative label
  • Marco De Spirito1; Roberto Brunelli2; Giampiero Mei3; Francesca R. Bertani4; Gabriele Ciasca1; Giulia Greco4; Massimiliano Papi1; Giuseppe Arcovito1; Fulvio Ursini5; Tiziana Parasassi4 (2006)
    Low density lipoprotein aged in plasma forms clusters resembling subendothelial droplets: aggregation via surface sites.
    in Biophysical journal (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Marco De Spirito1; Roberto Brunelli2; Giampiero Mei3; Francesca R. Bertani4; Gabriele Ciasca1; Giulia Greco4; Massimiliano Papi1; Giuseppe Arcovito1; Fulvio Ursini5; Tiziana Parasassi4 (literal)
Pagina inizio
  • 4239 (literal)
Pagina fine
  • 4247 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 90 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 11 (literal)
Note
  • ISI Web of Science (WOS) (literal)
  • PubMe (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1 Istituto di Neurobiologia e Medicina Molecolare, CNR, Rome, Italy; 1 Istituto di Fisica, Facolta` di Medicina e Chirurgia, Universita` Cattolica del Sacro Cuore, Rome, Italy; 2 Dipartimento di Scienze Ginecologiche, Perinatologia e Puericultura, Universita` di Roma ''La Sapienza'', Rome, Italy; 3 Dipartimento di Medicina Sperimentale e Scienze Biochimiche, Universita` di Roma ''Tor Vergata'', Rome, Italy; 4 Istituto di Neurobiologia e Medicina Molecolare, Consiglio Nazionale delle Ricerche, Rome, Italy; 5 Dipartimento di Chimica Biologica, Universita` di Padova, Padua, Italy (literal)
Titolo
  • Low density lipoprotein aged in plasma forms clusters resembling subendothelial droplets: aggregation via surface sites. (literal)
Abstract
  • In early phases of atherogenesis, droplets and vesicles accumulate in the subendothelial extracellular space of arterial intima. There is much evidence to suggest that these droplets, ranging between 100 and 400 nm, derive from modified low-density lipoprotein (LDL). In investigations of the formation mechanism of these droplets, LDL fusion was previously induced in vitro by proteolysis, lipolysis, oxidation, and vigorous shaking, but all treatments failed to reproduce the size distribution range of in vivo droplets, mostly resulting, instead, in particles with a diameter intermediate between that of one and two LDL. Our approach was meant to mimic LDL aging in plasma. LDL isolated from plasma that was incubated overnight at 37 degrees C is slightly modified in the secondary structure of its protein component and is primed to form very large aggregates according to a reaction-limited mechanism. This mechanism requires interactions between selected surface sites, whereas massive fusion is ruled out. In the frame of the general theory for colloids, the aggregation of LDL aged in plasma fulfills all the requirements of the reaction-limited mechanism, encompassing 1), exponential growth; 2), fractal structure, with the dimension of elementary constituent still consistent with a single LDL; and 3), extreme polydispersity of aggregates, with shape and dimension very close to that of droplets observed in vivo. (literal)
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