A novel pathway of cell growth regulation mediated by a PLA2{alpha}-derived phosphoinositide metabolite. (Articolo in rivista)

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  • A novel pathway of cell growth regulation mediated by a PLA2{alpha}-derived phosphoinositide metabolite. (Articolo in rivista) (literal)
Anno
  • 2006-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1096/fj.05-5397fje (literal)
Alternative label
  • Mariggio S., Sebastia J., Filippi B.M., Iurisci C., Volontè C., Amadio S., De Falco V., Santoro M., Corda D. (2006)
    A novel pathway of cell growth regulation mediated by a PLA2{alpha}-derived phosphoinositide metabolite.
    in The FASEB journal (Online)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Mariggio S., Sebastia J., Filippi B.M., Iurisci C., Volontè C., Amadio S., De Falco V., Santoro M., Corda D. (literal)
Pagina inizio
  • 2567 (literal)
Pagina fine
  • 2569 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni
  • IF = 6.721 SCI-JCR 2006 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 20 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 14 (literal)
Note
  • ISI Web of Science (WOS) (literal)
  • PubMe (literal)
  • Scopu (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Department of Cell Biology and Oncology, Consorzio Mario Negri Sud, Santa Maria Imbaro, Italy; *Santa Lucia Foundation/CNR, Rome, Italy; Dipartimento di Biologia e Patologia Cellulare e Molecolare, Università Federico II di Napoli, c/o Istituto di Endocrinologia e Oncologia Sperimentale del CNR, Naples, Italy. (literal)
Titolo
  • A novel pathway of cell growth regulation mediated by a PLA2{alpha}-derived phosphoinositide metabolite. (literal)
Abstract
  • The phosphoinositides have well-defined roles in the control of cellular functions, including cytoskeleton dynamics, membrane trafficking, and cell signaling. However, the interplay among the phosphoinositides and their diffusible derivatives that originate through phospholipase A2 action (the lysophosphoinositides and glycerophosphoinositols) remains to be fully elucidated. Here we demonstrate that in PCCl3 rat thyroid cells, the intracellular levels of glycerophosphoinositol are finely modulated by ATP and norepinephrine through the P2Y metabotropic and alpha-adrenergic receptors, respectively. The enzyme involved here is phospholipase A2 IValpha (PLA2IValpha), which in these cells specifically hydrolyzes phosphatidylinositol, forming lysophosphatidylinositol, glycerophosphoinositol, and arachidonic acid. This receptor-mediated activation of PLA2IValpha leads to stimulation of PCCl3 cell growth. The involvement of a PLA2IValpha-mediated pathway is demonstrated by inhibition of the increase in intracellular glycerophosphoinositol levels and cell proliferation by specific inhibitors, RNA interference, and overexpression of the dominant-negative PLA2IValpha1-522. Modulation of PCCl3 cell growth is not seen with inhibitors of arachidonic acid metabolism. In conclusion, these data characterize glycerophosphoinositol as a mediator of the purinergic and adrenergic regulation of PCCl3 cell proliferation, defining a novel regulatory cascade specifically involving this soluble phosphoinositide derivative and widening the involvement of the phosphoinositides in the regulation of cell function. (literal)
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