Gene expression profiling of human macrophages at late time of infection with Mycobacterium tuberculosis. (Articolo in rivista)

Type
Label
  • Gene expression profiling of human macrophages at late time of infection with Mycobacterium tuberculosis. (Articolo in rivista) (literal)
Anno
  • 2006-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1111/j.1365-2567.2006.02378.x (literal)
Alternative label
  • Volpe E.1; Cappelli G.2; Grassi M.3; Martino A.4; Serafino A.5; Colizzi V.6; Sanarico N.7; Mariani F.8. (2006)
    Gene expression profiling of human macrophages at late time of infection with Mycobacterium tuberculosis.
    in Immunology (Oxf., Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Volpe E.1; Cappelli G.2; Grassi M.3; Martino A.4; Serafino A.5; Colizzi V.6; Sanarico N.7; Mariani F.8. (literal)
Pagina inizio
  • 449 (literal)
Pagina fine
  • 460 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni
  • Impact Factor = 3.674 E-pub ahead of print 12 May 2006 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 118 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 4 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1,6,7 = Department of Biology, University of Rome 'Tor Vergata'; 1,2,3,5,7,8 = Institute of Neurobiology and Molecular Medicine, National Research Council; 4 = Unit of Cellular Immunology, National Institute for Infectious Diseases 'Lazzaro Spallanzani', IRCCS, Rome, Italy. (literal)
Titolo
  • Gene expression profiling of human macrophages at late time of infection with Mycobacterium tuberculosis. (literal)
Abstract
  • Macrophages play an essential role in the immune response to Mycobacterium tuberculosis (Mtb). Previous transcriptome surveys, by means of micro- and macroarrays, investigated the cellular gene expression profile during the early phases of infection (within 48 hr). However, Mtb remains within the host macrophages for a longer period, continuing to influence the macrophage gene expression and, consequently, the environment in which it persists. Therefore, we studied the transcription patterns of human macrophages for up to 7 days after infection with Mtb. We used a macroarray approach to study 858 human genes involved in immunoregulation, and we confirmed by quantitative real-time reverse transcriptase polymerase chain reaction (q-rt RT-PCR) and by enzyme-linked immunosorbent assay the most relevant modulations. We constantly observed the up-regulation in infected macrophages versus uninfected, of the following genes: interleukin-1 beta and interleukin-8, macrophage inflammatory protein-1 alpha, growth-related oncogene-beta, epithelial cell-derived neutrophil-activating peptide-78, macrophage-derived chemokine, and matrix metalloproteinase-7; whereas macrophage colony-stimulating factor-receptor and CD4 were down-regulated in infected macrophages. Mtb is able to withstand this intense cytokine microenvironment and to survive inside the human macrophage. Therefore we simultaneously investigated by q-rt RT-PCR the modulation of five mycobacterial genes: the alternative sigma factors sigA, sigE and sigG, the alpha-crystallin (acr) and the superoxide dismutase C (sodC) involved in survival mechanisms. The identified host and mycobacterial genes that were expressed until 7 days after infection, could have a role in the interplay between the host immune defences and the bacterial escape mechanisms. (literal)
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