http://www.cnr.it/ontology/cnr/individuo/prodotto/ID46603
Overexpression of a Grape Stilbene Synthase Gene in Tomato Induces Parthenocarpy And Causes Abnormal Pollen Development. (Articolo in rivista)
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- Label
- Overexpression of a Grape Stilbene Synthase Gene in Tomato Induces Parthenocarpy And Causes Abnormal Pollen Development. (Articolo in rivista) (literal)
- Anno
- 2011-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1016/j.plaphy.2011.07.012 (literal)
- Alternative label
Ingrosso I., Bonsegna S., De Domenico S., Laddomada B., Blando F., Santino A., Giovinazzo G. (2011)
Overexpression of a Grape Stilbene Synthase Gene in Tomato Induces Parthenocarpy And Causes Abnormal Pollen Development.
in Plant physiology and biochemistry (Paris)
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- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Ingrosso I., Bonsegna S., De Domenico S., Laddomada B., Blando F., Santino A., Giovinazzo G. (literal)
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- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
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- Titolo
- Overexpression of a Grape Stilbene Synthase Gene in Tomato Induces Parthenocarpy And Causes Abnormal Pollen Development. (literal)
- Abstract
- A novel strategy to induce parthenocarpy in tomato fruits by the induction of resveratrol biosynthesis in
flower tissues was exploited. Two transgenic tomato lines were considered: a higher resveratrolproducing
(35SS) line, constitutively expressing a grape stilbene synthase cDNA, and a lower resveratrolproducing
(LoxS) line, expressing stilbene synthase under a fruit-specific promoter. The expression of the
stilbene synthase gene affected flavonoid metabolism in a different manner in the transgenic lines, and in
one of these, the 35SS line, resulted in complete male sterility. Resveratrol was synthesised either in 35SS
or LoxS tomato flowers, at an even higher extent (about 8e10 times) in the former line. We further
investigated whether stilbene synthase expression may have resulted in impaired naringenin accumulation
during flower development. In the 35SS flowers, naringenin was significantly impaired by about
50%, probably due to metabolic competition. Conversely, the amount of glycosylated flavonols increased
in transgenic flowers, thereby excluding the diminished production of flavonols as a reason for
parthenocarpy in tomato. We further investigated whether resveratrol synthesis may have resulted
changes to pollen structure. Microscopic observations revealed the presence of few and abnormal flakelike
pollen grains in 35SS flowers with no germination capability. Finally, the analysis of coumaric and
ferulic acids, the precursors of lignin and sporopollenin biosynthesis, revealed significant depletion of
these compounds, therefore suggesting an impairment in structural compounds as a reason for pollen
ablation. These overall outcomes, to the best of our knowledge, reveal for the first time the major role
displayed by resveratrol synthesis on parthenocarpy in tomato fruits. (literal)
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