Effects of the aglycone of ascaulitoxin on amino acid metabolism in Lemna paucicostata. (Articolo in rivista)

Type
Label
  • Effects of the aglycone of ascaulitoxin on amino acid metabolism in Lemna paucicostata. (Articolo in rivista) (literal)
Anno
  • 2011-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.pestbp.2011.02.002 (literal)
Alternative label
  • Duke S.O., Evidente A., Fiore M., Rimando A.M., Dayan F.E., Vurro M., Christiansen N., Looser R., Hutzler J., Grossmann K. (2011)
    Effects of the aglycone of ascaulitoxin on amino acid metabolism in Lemna paucicostata.
    in Pesticide biochemistry and physiology
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Duke S.O., Evidente A., Fiore M., Rimando A.M., Dayan F.E., Vurro M., Christiansen N., Looser R., Hutzler J., Grossmann K. (literal)
Pagina inizio
  • 41 (literal)
Pagina fine
  • 50 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 100 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • USDA-ARS-NPURU, P.O. Box 8048, University, MS 38677, USA Dipartimento di Scienze del Suolo, della Pianta, dell'Ambiente e delle Produzioni Animali, Università di Napoli Federico II, Via Università 100, 80055 Portici, Italy Istituto di Scienze delle Produzioni Alimentari, CNR, Via Amendola 122/O, 70125 Bari, Italy Metanomics GmbH, D-10589 Berlin, Germany BASF Agricultural Center, D-67117 Limburgerhof, Germany (literal)
Titolo
  • Effects of the aglycone of ascaulitoxin on amino acid metabolism in Lemna paucicostata. (literal)
Abstract
  • Ascaulitoxin and its aglycone (2,4,7-triamino-5-hydroxyoctanoic acid, CAS 212268-55-8) are potent phytotoxins produced by Ascochyta caulina, a plant pathogen being developed for biocontrol of weeds. The mode of action of this non-protein amino acid was studied on Lemna paucicostata. Ascaulitoxin is a potent growth inhibitor, with an I50 for growth of less than 1 lM, almost completely inhibiting growth at about 3 lM. Its action is slow, starting with growth inhibition, followed by darker green fronds, and then chlorosis and death. Most amino acids, including non-toxic non-protein amino acids, reversed the effect of the toxin when supplemented in the same medium. Supplemental sucrose slightly increased the activity. D-Amino acids were equally good inhibitors of ascaulitoxin activity, indicating the amino acid effects may not be due to inhibition of amino acid synthesis. Oxaloacetate, the immediate precursor of aspartate, also reversed the activity. LC-MS did not detect interaction of the compound with lysine, an amino acid that strongly reversed the effect of the phytotoxin. Metabolite profiling revealed that the toxin caused distinct changes in amino acids. Reduction in alanine, paralleled by enhanced levels of the branched chain amino acids valine, leucine and isoleucine and nearly unchanged levels of pyruvate, might indicate that the conversion of pyruvate to alanine is affected by ascaulitoxin aglycone. In addition, reduced levels of glutamate/glutamine and aspartate/asparagine might suggest that synthesis and interconversion reactions of these amino group donors are affected. However, neither alanine aminotransferase nor alanine: glyoxylate aminotransferase were inhibited by the toxin in vitro. Our observations might be explained by three hypotheses: (1) the toxin inhibits one or more aminotransferases not examined, (2) ascaulitoxin aglycone affects amino acid transporters, (3) ascaulitoxin aglycone is a protoxin that is converted in vivo to an aminotransferase inhibitor. (literal)
Prodotto di
Autore CNR

Incoming links:


Prodotto
Autore CNR di
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi
data.CNR.it