Feasibilty of in utero DNA vaccination following naked gene transfer into pig fetal muscle: Transgene expression, immunity and safety (Articolo in rivista)

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  • Feasibilty of in utero DNA vaccination following naked gene transfer into pig fetal muscle: Transgene expression, immunity and safety (Articolo in rivista) (literal)
Anno
  • 2006-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.vaccine.2005.08.030 (literal)
Alternative label
  • Monica Rinaldi a; Emanuela Signori a, b; Paolo Rosati c; Giorgio Cannelli d; Paola Parrella e; Enrico Iannace d; Giovanni Monego c; Silvia Anna Ciafrè f; Maria Giulia Farace f; Sandra Iurescia a,e; Daniela Fioretti a,e; Guido Rasi a; Vito Michele Fazio a,b,e; (2006)
    Feasibilty of in utero DNA vaccination following naked gene transfer into pig fetal muscle: Transgene expression, immunity and safety
    in Vaccine
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Monica Rinaldi a; Emanuela Signori a, b; Paolo Rosati c; Giorgio Cannelli d; Paola Parrella e; Enrico Iannace d; Giovanni Monego c; Silvia Anna Ciafrè f; Maria Giulia Farace f; Sandra Iurescia a,e; Daniela Fioretti a,e; Guido Rasi a; Vito Michele Fazio a,b,e; (literal)
Pagina inizio
  • 4586 (literal)
Pagina fine
  • 4591 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni
  • KeyWords Plus: PLASMID DNA; INTRAMUSCULAR INJECTION; SKELETAL-MUSCLE; MICE; IMMUNIZATION; THERAPY; SHEEP; LUCIFERASE; MEMORY Publisher: ELSEVIER SCI LTD, THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND Web of Science Category: Immunology; Medicine, Research & Experimental Subject Category: Immunology; Research & Experimental Medicine IDS Number: 056JI ISSN: 0264-410X (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 24 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 21 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • a Institute of Neurobiology and Molecular Medicine, CNR, Via Fosso del Cavaliere 100, 00133 Rome, Italy; b CIR, Section of Molecular Medicine and Biotechnology, University \"Campus Bio-Medico\", Via E. Longoni 83, 00155 Rome, Italy; c Institutes of Obstetrics and Gynecology, Università Cattolica del Sacro Cuore (UCSC), Largo Francesco Vito 1, 00168 Rome, Italy; d Anaesthesiology and Reanimation, Università Cattolica del Sacro Cuore (UCSC), Largo Francesco Vito 1, 00168 Rome, Italy; e Laboratory for Oncology and Molecular Pathology of Aging, IRCCS H. \"Casa Sollievo della Sofferenza\", Viale dei Cappuccini, 71013 San Giovanni Rotondo (FG), Italy; f Department of Experimental Medicine and Biochemical Sciences, University of Rome Tor Vergata, Università di Roma \"Tor Vergata\", Via di Tor Vergata 135, 00133 Rome, Italy (literal)
Titolo
  • Feasibilty of in utero DNA vaccination following naked gene transfer into pig fetal muscle: Transgene expression, immunity and safety (literal)
Abstract
  • The high toll of death among first-week infants is due to infections occurring at the end of pregnancy, during birth or by breastfeeding. This problem significantly concerns industrialized countries also. To prevent the typical \"first-week infections\", a vaccine would be protective as early as at the birth. In utero DNA immunization has demonstrated the effectiveness in inducing specific immunity in newborns. We have already published results of a 2-year follow-up showing long-term safety, protective antibody titers at birth and long-term immune memory, following intramuscular in utero anti-HBV DNA immunization in 90-days pig fetuses. We have now analyzed further parameters of short-term safety. Two different reporter genes were injected in the thigh muscles of 90-days fetuses. At 8 days following DNA injection, we found high-level of transgenes expression in all injected fetuses. A step gradient of expression from the area of injection was observed with both reporter genes. CMV promoter/enhancer produced higher levels of expression compared to SV40 promoter/enhancer. Moreover, no evidence of local or systemic flogistic alterations or fetal malformations, mortality or haemorrhage following intramuscular injection were observed. A single anti-HBV s-antigen DNA immunization in 90-days fetuses supported protective antibody levels in all immunized newborns, lasting at least up to 4 months after birth. Our report further sustains safety and efficacy of intramuscular in utero naked gene transfer and immunization. This approach may support therapeutic or prophylactic procedure in many early life-threatening pathologic conditions. (literal)
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