Expression of LINE-1 retroposons is essential for murine preimplantation development. (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Expression of LINE-1 retroposons is essential for murine preimplantation development. (Articolo in rivista) (literal)

Anno

• 2006-01-01T00:00:00+01:00 (literal)

Alternative label

• Beraldi R.1, Pittoggi C.2, Sciamanna I.3, Mattei E.4, Spadafora C.5 (2006)
  Expression of LINE-1 retroposons is essential for murine preimplantation development.
  in Molecular reproduction and development
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Beraldi R.1, Pittoggi C.2, Sciamanna I.3, Mattei E.4, Spadafora C.5 (literal)

Pagina inizio

• 279 (literal)

Pagina fine

• 287 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni

• Impact Factor = 2.379 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 73/3 (literal)

Rivista

• Molecular reproduction and development (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1 = University of Siena, Siena, Italy; 1,2,3,5 = Istituto Superiore di Sanità (ISS), Rome, Italy; 4 = CNR, National Research Council, Rome, Italy. (literal)

Titolo

• Expression of LINE-1 retroposons is essential for murine preimplantation development. (literal)

Abstract

• In higher eukaryotes, reverse transcriptase (RT) activities are encoded by a variety of endogenous retroviruses and retrotransposable elements. We previously found that mouse preimplantation embryos are endowed with an endogenous RT activity. Inhibition of that activity by the non nucleosidic inhibitor nevirapine or by microinjection of anti-RT antibody caused early embryonic developmental arrest. Those experiments indicated that RT is required for early development, but did not identify the responsible coding elements. We now show that microinjection of morpholino-modified antisense oligonucleotides targeting the 5 end region of active LINE-1 retrotransposons in murine zygotes irreversibly arrests preimplantation development at the two- and four-cell stages; the overall level of functional RT is concomitantly downregulated in arrested embryos. Furthermore, we show that the induction of embryo developmental arrest is associated with a substantial reprogramming of gene expression. Together, these results support the conclusion that expression of LINE-1 retrotransposons is required for early embryo preimplantation development. (literal)

Prodotto di

• Progettazione e realizzazione di modelli animali per lo studio e la terapia di patologie del S.N. (ME.P02.010.002) (Modulo)
• Istituto di neurobiologia e medicina molecolare (INMM) (Istituto)

Autore CNR

• ELISABETTA MATTEI (Persona)

Insieme di parole chiave

• Keywords of "Expression of LINE-1 retroposons is essential for murine preimplantation development." (Insieme di parole chiave)

Incoming links:

Prodotto

• Istituto di neurobiologia e medicina molecolare (INMM) (Istituto)
• Progettazione e realizzazione di modelli animali per lo studio e la terapia di patologie del S.N. (ME.P02.010.002) (Modulo)

Autore CNR di

• ELISABETTA MATTEI (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Molecular reproduction and development (Rivista)

Insieme di parole chiave di

• Keywords of "Expression of LINE-1 retroposons is essential for murine preimplantation development." (Insieme di parole chiave)