Characterisation of a pks gene which is expressed during ochratoxin A production by Aspergillus carbonarius. (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Characterisation of a pks gene which is expressed during ochratoxin A production by Aspergillus carbonarius. (Articolo in rivista) (literal)

Anno

• 2009-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.ijfoodmicro.2008.10.022 (literal)

Alternative label

• Gallo A.; Perrone G.; Solfrizzo M.; Epifani F.; Abbas A.; Dobson A.D.W.; Mulè G. (2009)
  Characterisation of a pks gene which is expressed during ochratoxin A production by Aspergillus carbonarius.
  in International journal of food microbiology
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Gallo A.; Perrone G.; Solfrizzo M.; Epifani F.; Abbas A.; Dobson A.D.W.; Mulè G. (literal)

Pagina inizio

• 8 (literal)

Pagina fine

• 15 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni

• IF 3.155 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 129 (literal)

Rivista

• International journal of food microbiology (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• (1) Institute of Sciences of Food Production, ISPA-CNR, Via Amendola 122/0, 70126, Bari, ITALY (2) Department of Microbiology and Environmental Research Institute, University College Cork, National University of Ireland, Lee Road, Cork, IRLANDE (literal)

Titolo

• Characterisation of a pks gene which is expressed during ochratoxin A production by Aspergillus carbonarius. (literal)

Abstract

• Aspergillus carbonarius is considered the main fungus responsible for ochratoxin A (OTA) contamination in grapes. OTA is a potent nephrotoxin and a possible human carcinogen with a polyketide derived structure. Fungal polyketide synthases (PKSs) have recently been demonstrated to be involved in OTA biosynthesis in both Penicillium and Aspergillus species. We report here on the identification and characterisation of part of a novel polyketide synthase gene, ACpks from A. carbonarius. The sequence appears to encode conserved ketosynthase and acyl transferase domains, which are characteristic of previously characterised PKS enzymes. Expression of the ACpks gene is differentially regulated, with transcription being observed when the fungus was grown on synthetic grape medium and on OTA permissive medium (MM) whereas no transcription was detected when the fungus was grown on OTA restrictive medium (YES). ACpks expression was also observed when A. carbonarius was grown at low pH, with concomitant increases in OTA production. This correlation between ACpks gene expression and OTA production suggests the likely involvement for the product of this gene in ochratoxin A biosynthesis in the fungus. From a preliminary screening of Aspergillus isolates with ACpks specific primers, ACpks gene homologues appear to be present in A. sclerotioniger and A. ibericus, two species of section Nigri which are closely related to A. carbonarius. (literal)

Prodotto di

• Istituto di scienze delle produzioni alimentari (ISPA) (Istituto)
• Metodi innovativi per l'analisi e la riduzione di micotossine, funghi tossigeni ed allergeni nei prodotti agroalimentari (AG.P05.008.002) (Modulo)

Autore CNR

• GIANCARLO PERRONE (Persona)
• FILOMENA EPIFANI (Unità di personale interno)
• GIUSEPPINA MULE' (Persona)
• MICHELE SOLFRIZZO (Unità di personale interno)
• ANTONIA GALLO (Persona)

Insieme di parole chiave

• Parole chiave di "Characterisation of a pks gene which is expressed during ochratoxin A production by Aspergillus carbonarius." (Insieme di parole chiave)

Incoming links:

Autore CNR di

• FILOMENA EPIFANI (Unità di personale interno)
• GIANCARLO PERRONE (Persona)
• MICHELE SOLFRIZZO (Unità di personale interno)
• GIUSEPPINA MULE' (Persona)
• ANTONIA GALLO (Persona)

Prodotto

• Istituto di scienze delle produzioni alimentari (ISPA) (Istituto)
• Metodi innovativi per l'analisi e la riduzione di micotossine, funghi tossigeni ed allergeni nei prodotti agroalimentari (AG.P05.008.002) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• International journal of food microbiology (Rivista)

Insieme di parole chiave di

• Parole chiave di "Characterisation of a pks gene which is expressed during ochratoxin A production by Aspergillus carbonarius." (Insieme di parole chiave)