http://www.cnr.it/ontology/cnr/individuo/prodotto/ID46401
A filtration-based real-time PCR method for the quantitative detection of viable Salmonella enterica and Listeria monocytogenes in food samples. (Articolo in rivista)
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- Label
- A filtration-based real-time PCR method for the quantitative detection of viable Salmonella enterica and Listeria monocytogenes in food samples. (Articolo in rivista) (literal)
- Anno
- 2009-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1016/j.fm.2008.12.006 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- D'Urso OF; Poltronieri P; Marsigliante S; Storelli C; Hernández M; Rodríguez-Lázaro D (literal)
- Pagina inizio
- Pagina fine
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- Impact Factor 3.374
Marie Curie Grant MERG CT 2007 209050 (literal)
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- http://www.scopus.com/record/display.url?eid=2-s2.0-61449145431&origin=resultslist&sort=plf-f&src=s&sid=11A559D9D7402050FBAF68E81086B107.f594dyPDCy4K3aQHRor6A%3a140&sot=autdocs&sdt=autdocs&sl=17&s=AU-ID%286603277221%29&relpos=25&relpos=5&citeCnt=25&searchTerm= (literal)
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- Department of Environmental and Biological Sciences, Institute of Physiology, University of Salento, Lecce, Italy
Food Safety and Technology Research Group, Instituto Tecnologico Agrario de Castilla y Leon (ITACyL), Valladolid, Spain
Institute of Sciences of Food Productions, National Research Council (ISPA-CNR) (literal)
- Titolo
- A filtration-based real-time PCR method for the quantitative detection of viable Salmonella enterica and Listeria monocytogenes in food samples. (literal)
- Abstract
- We developed a novel filtration-based method that can eliminate dead or severally damaged Salmonella enterica and Listeria monocytogenes in food samples. This new method can recover all viable bacteria in less than 30 min, and can be coupled with a subsequent bacterial DNA extraction and real-time PCR. No statically significant differences (p < 0.01) were found between real-time PCR results obtained separately from S. enterica and L. monocytogenes when different ratios of living and dead cells were used. The analytical sensitivity in both cases was 1 genome equivalent (GE), and the quantification was linear (R2 > 0.9969) over a 5-log dynamic range with PCR efficiencies >0.9754. When compared with the standard microbiological methods for the detection of these foodborne pathogens, the relative accuracy was excellent ranging from 95.72% to 104.48%. Finally, we applied the pre-treatment method to the direct detection of viable forms of these foodborne pathogens in food samples using yogurt as a model, the results being similar to those obtained using pure cultures. (literal)
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