An improved, real-time PCR assay for the detection of GC-rich and low abundance templates of Mycobacterium tuberculosis (Articolo in rivista)

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Label
  • An improved, real-time PCR assay for the detection of GC-rich and low abundance templates of Mycobacterium tuberculosis (Articolo in rivista) (literal)
Anno
  • 2006-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.mimet.2005.05.002 (literal)
Alternative label
  • Grassi M.1, Volpe E.2, Colizzi V.3, Mariani F.4 (2006)
    An improved, real-time PCR assay for the detection of GC-rich and low abundance templates of Mycobacterium tuberculosis
    in Journal of microbiological methods
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Grassi M.1, Volpe E.2, Colizzi V.3, Mariani F.4 (literal)
Pagina inizio
  • 406 (literal)
Pagina fine
  • 410 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni
  • Impact Factor = 2.442 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 64 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 3 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1,2,4 = Institute Neurobiology and Molecular Medicine, Molec. Med. Section, National Research Council, Via del Fosso del Cavaliere, 100, 00133, Rome, Italy; 2,3 = Deparment Biology, University of Rome “Tor Vergata”, Rome, Italy. (literal)
Titolo
  • An improved, real-time PCR assay for the detection of GC-rich and low abundance templates of Mycobacterium tuberculosis (literal)
Abstract
  • The detection of low abundance mRNA and/or GC-rich targets is very difficult using real-time PCR, often requiring laborious optimization procedures. This work shows that formamide is a useful PCR additive, increasing the sensitivity and specificity of SYBR Green real-time PCR to detect low abundance mycobacterial RNA from infected samples. (literal)
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