Combined cup loading, bis(2-hydroxyethyl)disulfide, and protein precipitation protocols to improve the alkaline proteome of Lactobacillus hilgardii. (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Combined cup loading, bis(2-hydroxyethyl)disulfide, and protein precipitation protocols to improve the alkaline proteome of Lactobacillus hilgardii. (Articolo in rivista) (literal)

Anno

• 2007-01-01T00:00:00+01:00 (literal)

Alternative label

• Lamberti C., Pessione E., Giuffrida M.G., Mazzoli R., Barello C., Conti A., Giunta C. (2007)
  Combined cup loading, bis(2-hydroxyethyl)disulfide, and protein precipitation protocols to improve the alkaline proteome of Lactobacillus hilgardii.
  in Electrophoresis (Weinh., Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Lamberti C., Pessione E., Giuffrida M.G., Mazzoli R., Barello C., Conti A., Giunta C. (literal)

Pagina inizio

• 1633 (literal)

Pagina fine

• 1638 (literal)

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• 28 (literal)

Rivista

• Electrophoresis (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto

• Ricerca di carattere metodologico per una migliore separazione e caratterizzazione delle proteine basiche (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Maria Gabriella Giuffrida Cristina Barello, Amedeo Conti - ISPA-CNR c/o Bioindustry Park Canavese. Colleretto Giacosa (To), Italy; Cristina Lamberti, Enrica Pessione, Roberto Mazzoli, Carlo Giunta - Dipartimento di Biologia Animale e dell’Uomo, Università di Torino, Italy. (literal)

Titolo

• Combined cup loading, bis(2-hydroxyethyl)disulfide, and protein precipitation protocols to improve the alkaline proteome of Lactobacillus hilgardii. (literal)

Abstract

• Despite the large number of papers dealing with bacterial proteomes, very few include information about proteins with alkaline isoelectric points, because of the limits inherent in 2DE technology. Nonetheless, analyses of in silico proteomes of many prokaryotes show a bimodal distribution of their proteins based on their pIs, the most crowded areas lying between pI 4-7 and pI 9-11. The aim of the present research was to set up a general, simple and standardizable 2DE protocol suitable for studying alkaline proteome of L. hilgardii, a Gram-positive bacillus isolated from wine. The method has been also tested on a Gram-negative bacterium able to degrade aromatic pollutants, A. radioresistens S13. Optimization of the method was mainly focused on improving protein extraction and IEF (pI 6-11) separation protocols. Concerning IEF, different methods for sample loading (in-gel rehydration and cup-loading), and different reducing agents (DTT and HED) were tested and compared. The proposed protocol was found to resolve alkaline proteins from both our Lactobacillus and Acinetobacter strains efficiently, in spite of their different external layers, thus enabling more ....................... (literal)

Prodotto di

• Istituto di scienze delle produzioni alimentari (ISPA) (Istituto)
• Metodi innovativi per l'analisi e la riduzione di micotossine, funghi tossigeni ed allergeni nei prodotti agroalimentari (AG.P05.008.002) (Modulo)

Autore CNR

• CRISTINA BARELLO (Unità di personale esterno)
• CRISTINA LAMBERTI (Persona)
• AMEDEO CONTI (Persona)
• MARIA GABRIELLA GIUFFRIDA (Persona)

Insieme di parole chiave

• Keywords of "Combined cup loading, bis(2-hydroxyethyl)disulfide, and protein precipitation protocols to improve the alkaline proteome of Lactobacillus hilgardii." (Insieme di parole chiave)

Incoming links:

Prodotto

• Istituto di scienze delle produzioni alimentari (ISPA) (Istituto)
• Metodi innovativi per l'analisi e la riduzione di micotossine, funghi tossigeni ed allergeni nei prodotti agroalimentari (AG.P05.008.002) (Modulo)

Autore CNR di

• MARIA GABRIELLA GIUFFRIDA (Persona)
• AMEDEO CONTI (Persona)
• CRISTINA BARELLO (Unità di personale esterno)
• CRISTINA LAMBERTI (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Electrophoresis (Rivista)

Insieme di parole chiave di

• Keywords of "Combined cup loading, bis(2-hydroxyethyl)disulfide, and protein precipitation protocols to improve the alkaline proteome of Lactobacillus hilgardii." (Insieme di parole chiave)