http://www.cnr.it/ontology/cnr/individuo/prodotto/ID46150
Development of multiplex PCR assay for the identification of Staphylococcus aureus enterotoxigenic strains isolated from milk and dairy products. (Articolo in rivista)
- Type
- Label
- Development of multiplex PCR assay for the identification of Staphylococcus aureus enterotoxigenic strains isolated from milk and dairy products. (Articolo in rivista) (literal)
- Anno
- 2005-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1016/j.mcp.2005.03.002 (literal)
- Alternative label
Paola Cremonesi; Massimo Luzzana; Milena Brasca; Stefano Morandi; Roberta Lodi; Chiara Vimercati; Dario Agnellini; Giancarlo Caramenti; Paolo Moroni; Bianca Castiglioni (2005)
Development of multiplex PCR assay for the identification of Staphylococcus aureus enterotoxigenic strains isolated from milk and dairy products.
in Molecular and cellular probes (Print); Academic Press, New York (Stati Uniti d'America)
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Paola Cremonesi; Massimo Luzzana; Milena Brasca; Stefano Morandi; Roberta Lodi; Chiara Vimercati; Dario Agnellini; Giancarlo Caramenti; Paolo Moroni; Bianca Castiglioni (literal)
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- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
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- ISI Web of Science (WOS) (literal)
- Scopus (literal)
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- Google Scholar (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Milena Brasca, Stefano Morandi, Roberta LodiCNR - ISPA Milano; Bianca Castiglioni -CNR IBBA,
Paola Cremonesi, Massimo Luzzana, Chiara Vimercati, Dario Agnellini, Paolo Moroni - Università di Milano; Giancarlo Caramenti-ITB-CNR (literal)
- Titolo
- Development of multiplex PCR assay for the identification of Staphylococcus aureus enterotoxigenic strains isolated from milk and dairy products. (literal)
- Abstract
- A multiplex PCR for the simultaneous detection of Staphylococcus aureus 23S rRNA, the coagulase and thermonuclease genes as well as the enterotoxin genes sea, sec, sed, seg, seh, sei, sej, sel was developed. The method was used to determine the presence of enterotoxigenic types for 93 S. aureus strains isolated from milk and dairy products. The data obtained by mPCR resulted comparable to those obtained by immunoassay methods. In addition, the mPCR assays also amplified some se genes, whose toxins are undetectable by immunoassay. Multiplex amplification can be obtained starting from 1 pg of DNA, showing the excellent specificity and high sensitivity of the assay. (literal)
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