http://www.cnr.it/ontology/cnr/individuo/prodotto/ID4614

AMPA receptors are modulated by tachykinins in rat cerebellum neurons. (Articolo in rivista)

Type

Articolo in rivista (Classe)
Prodotto della ricerca (Classe)

Label

AMPA receptors are modulated by tachykinins in rat cerebellum neurons. (Articolo in rivista) (literal)

Anno

2005-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

10.1152/jn.00436.2005 (literal)

Alternative label

Massimo Pieri,1,2; Cinzia Severini,3; Giuseppina Amadoro,3 ; Irene Carunchio,1,2 ; Christian Barbato,3; Maria Teresa Ciotti,3; and Cristina Zona1,2 (2005)
AMPA receptors are modulated by tachykinins in rat cerebellum neurons.
in Journal of neurophysiology
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Massimo Pieri,1,2; Cinzia Severini,3; Giuseppina Amadoro,3 ; Irene Carunchio,1,2 ; Christian Barbato,3; Maria Teresa Ciotti,3; and Cristina Zona1,2 (literal)

Pagina inizio

2484 (literal)

Pagina fine

2490 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni

Impact Factor = 3.853 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

94 (literal)

Rivista

Journal of neurophysiology (Rivista)

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4 (literal)

Note

ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

1Department of Neuroscience, University of Rome \"Tor Vergata\"; 2Istituto di Ricovero e Cura a Carattere Scientifico Fondazione Santa Lucia, Centro Europeo di Ricerca sul Cervello 3Institute of Neurobiology and Molecular Medicine, Rome, Italy (literal)

Titolo

AMPA receptors are modulated by tachykinins in rat cerebellum neurons. (literal)

Abstract

The peptides of the tachykinin family are widely distributed within the mammalian peripheral and central nervous systems and play a well-recognized role as neuromodulators, although their direct action on cerebellum granule cells have not yet been demonstrated. We have examined the effect of the best known members of the family, substance P (SP), neurokinin A (NKA), and neurokinin B (NKB) on alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors from rat cerebellar granule cells in culture to assess the ability of these peptides to regulate the glutamatergic input. Both NKA and NKB, but not SP, produce a significant enhancement of ionic current through AMPA receptors activated by the agonist kainate in 53.5 and 46% of patched neurons, respectively. This effect was not observable in the presence of MEN 10,627 and Trp(7)betaAla(8), NKA and NKB competitive antagonist receptors, respectively, indicating that the current modulations were mediated by the respective receptors. NKB also produces a significant enhancement of ionic current through the AMPA receptors activated directly by its agonist AMPA and cyclothiazide, an allosteric modulator that selectively suppresses desensitization of AMPA receptors. The presence of NK3 receptors was demonstrated in these neurons by RT-PCR amplification of total RNA extracted from cerebellar granule cells, using NK3-specific primer pairs. Immunocytochemistry experiments, using a specific polyclonal antibody directed against NK3, also confirmed the presence of NK3 receptors and their co-localization with the GLUR2 AMPA subunit in about 54% of cerebellar granule neurons. This study adds the tachykinins to the list of neuromodulators capable of exerting a excitatory action on cerebellar granule cells. (literal)

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