9-Lipoxygenase metabolism is involved in the almond/Aspergillus carbonarius interaction. (Articolo in rivista)

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Label
  • 9-Lipoxygenase metabolism is involved in the almond/Aspergillus carbonarius interaction. (Articolo in rivista) (literal)
Anno
  • 2007-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1093/jxb/erm039 (literal)
Alternative label
  • Mita G., Fasano P., De Domenico S., Perrone G., Epifani F., Iannacone R., Casey R., Santino A. (2007)
    9-Lipoxygenase metabolism is involved in the almond/Aspergillus carbonarius interaction.
    in Journal of experimental botany
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Mita G., Fasano P., De Domenico S., Perrone G., Epifani F., Iannacone R., Casey R., Santino A. (literal)
Pagina inizio
  • 1803 (literal)
Pagina fine
  • 1811 (literal)
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  • 58 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto
  • In questo lavoro è stata studiata l’interazione tra semi di mandorle e l’infezione da Apergillus carbonarius. In particolare è stato analizzato l’effetto sulla via biosintetica delle lipossigenasi del seme in risposta all’infezione del patogeno verificando che la risposta è molto più elevata nei semi immaturi rispetto a quelli maturi. Infatti la produzione di aldeidi in grado di bloccare la crescita di A. carbonarius è stata identificata solo nelle mandorle immature. (literal)
Note
  • Scopu (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Giovanni Mita, Pasqua Fasano, Stefania De Domenico Angelo Santino Istituto di Scienze delle Produzioni Alimentari, Sezione di Lecce, Via Monteroni, 73100, Lecce Giancarlo Perrone, Filomena Epifani Istituto di Scienze delle Produzioni Alimentari, Via Amendola 122/O, 70126 Bari Rina Iannacone, Metapontum Agrobios, S.S. Jonica 106, Km 448.2, 75010, Metaponto (MT), Italy Rod Casey John Innes Centre, Norwich Research Park, Colney, Norwich, NR4 7UH, UK (literal)
Titolo
  • 9-Lipoxygenase metabolism is involved in the almond/Aspergillus carbonarius interaction. (literal)
Abstract
  • Phyto-oxylipins are a group of biologically active molecules that play an important role in plant defence. Their production begins with the oxygenation of polyunsaturated fatty acids by lipoxygenases (LOX) to form 9- or 13-hydroperoxides that are substrates for several enzymes involved in the synthesis of final oxylipins, which can act as signal molecules and/or direct antimicrobials. In the present work, the response of the 9-LOX pathway in the almond/Aspergillus carbonarius (producer of ochratoxin A) interaction was studied. Both LOX gene expression and activity are up-regulated over the course of fungal infection in immature and mature almonds. The biochemical characterization of major LOX and hydroperoxide lyase (HPL) isoforms indicated that 9-LOX metabolism is specifically induced by A. carbonarius. Lipid peroxidation profiling showed that, in infected immature almonds, enzymatically produced 9-hydro(peroxy) fatty acids (HFAs) were the main HFAs and are further metabolized by HPL into C9-aldehydes. Both HPL gene expression and C9-aldehydes increased over the course of fungal infection. In mature almonds infected with A. carbonarius, levels of LOX expression and activity were lower than those found in immature seeds, and 9-HFA represented the minority of total HFA, which consisted of mostly 13- and non-enzymatically produced HFA. In these experimental conditions, no volatile aldehydes were recorded from these samples, even though HPL was up-regulated in infected mature almonds. The effects on the growth of A. carbonarius of the aldehydes produced by these enzymes were also tested in vitro. Results reported here led to the proposal that, in almond seed, the association of 9-LOX and HPL has an important role in seed defence mechanism against pathogen infection (literal)
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