http://www.cnr.it/ontology/cnr/individuo/prodotto/ID4605

Anti-gene peptide nucleic acid targeted to proviral HIV-1 DNA inhibits in vitro HIV-1 replication. (Articolo in rivista)

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Anti-gene peptide nucleic acid targeted to proviral HIV-1 DNA inhibits in vitro HIV-1 replication. (Articolo in rivista) (literal)

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Pesce C.D.1, Bolacchi F.2, Bongiovanni B.3, Cisotta F.4, Capozzi M.5, Diviacco S.6, Quadrifoglio F.7, Mango R.8, Novelli G.9, Mossa G.10, Esposito C.11, Ombres D.12, Rocchi G.13, Bergamini A.14 (2005)
Anti-gene peptide nucleic acid targeted to proviral HIV-1 DNA inhibits in vitro HIV-1 replication.
in Antiviral research (Print)
(literal)

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Pesce C.D.1, Bolacchi F.2, Bongiovanni B.3, Cisotta F.4, Capozzi M.5, Diviacco S.6, Quadrifoglio F.7, Mango R.8, Novelli G.9, Mossa G.10, Esposito C.11, Ombres D.12, Rocchi G.13, Bergamini A.14 (literal)

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1,3,12 = Department of Experimental Medicine and Biochemical Sciences, University of Roma Tor Vergata, Rome, Italy; 2,4,5,13,14 = Department of Public Health and Cellular Biology, Chair of Infectious Diseases, University of Roma Tor Vergata, Rome, Italy; 6,7 = Department of Sciences and Biomedical Technologies, University of Udine, Udine, Italy; 8,9 = Department of Biopathology and Diagnostic Imaging, University of Roma Tor Vergata, Rome, Italy; 10,11 = Institute of Neurobiology and Molecular Medicine, National Council of Research (CNR) Rome, Italy. (literal)

Titolo

Anti-gene peptide nucleic acid targeted to proviral HIV-1 DNA inhibits in vitro HIV-1 replication. (literal)

Abstract

Highly active antiretroviral therapy (HAART) is unlikely to affect reservoirs of HIV in latently infected cells. Anti-gene compounds, such as peptide nucleic acids (PNAs), which block transcriptional activity via sequence-specific invasion of double-stranded DNA may be an effective strategy to target cells harbouring proviral HIV DNA. Here we show that a PNA oligomer (PNA(HIV)), 15 bases in length, linked to a nuclear localization signal (NLS), substantially suppressed HIV-1 replication in chronically infected lymphocytes and macrophages and efficiently prevented mitogen-induced HIV-1 reactivation in lymphocytes, as determined by HIV-p24 antigen production in supernatants and FACS analysis for intracellular HIV accumulation. In contrast, a mismatched PNA did not show any effect on HIV expression. Semi-quantitative RT-PCR and quantitative real-time RT-PCR demonstrated a decrease of HIV RNA expression in infected cells treated by PNA(HIV) indicating that inhibition of HIV-1 replication occurred at the transcription step. In conclusion, the use of anti-gene PNA to target the HIV-1 proviral DNA in the quest for new antiretroviral agents appears quite promising. (literal)

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