Transmission in vitro of hepatitis C virus from persistently infected human B-cells to hepatoma cells by cell-to-cell contact. (Articolo in rivista)

Type
Label
  • Transmission in vitro of hepatitis C virus from persistently infected human B-cells to hepatoma cells by cell-to-cell contact. (Articolo in rivista) (literal)
Anno
  • 2006-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1002/jmv.20527 (literal)
Alternative label
  • Valli M.B.1,4; Serafino A.L.1; Crema A.L.1; Bertolini L.1; Manzin A.2; Lanzilli G.1; Bosman C.3; Iacovacci S.1; Giunta S.4; Ponzetto A.5; Clementi M.6; Carloni G.1 (2006)
    Transmission in vitro of hepatitis C virus from persistently infected human B-cells to hepatoma cells by cell-to-cell contact.
    in Journal of medical virology (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Valli M.B.1,4; Serafino A.L.1; Crema A.L.1; Bertolini L.1; Manzin A.2; Lanzilli G.1; Bosman C.3; Iacovacci S.1; Giunta S.4; Ponzetto A.5; Clementi M.6; Carloni G.1 (literal)
Pagina inizio
  • 192 (literal)
Pagina fine
  • 201 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni
  • Impact Factor = 2.779 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 78 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 2 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1 =Institute of Neurobiology and Molecular Medicine, National Research Council (CNR), Area di Ricerca CNR TorVergata, Rome, Italy; 2 = Institute of Microbiology, University of Ancona, Ancona, Italy; 3 =Department of Pathology, Rome University La Sapienza, Roma, Italy; 4 =Laboratory of Clinical pathology, INRCA Geriatric Hospital, Ancona, Italy; 5 = Department of Internal Medicine, Torino University, Torino, Italy; 6 = Laboratory of Microbiology, Vita-Salute San Raffaele University, San Raffaele Institute, Milano, Italy. (literal)
Titolo
  • Transmission in vitro of hepatitis C virus from persistently infected human B-cells to hepatoma cells by cell-to-cell contact. (literal)
Abstract
  • Virus cell-to-cell spread has been reported for many different viruses and may contribute to pathogenesis of viral disease. The role played by cell-to-cell contact in hepatitis C virus (HCV) transmission was studied in vitro by cell co-cultivation experiments. A human lymphoblastoid B-cell line, infected persistently with HCV in vitro (TO.FEHCV), was used as HCV donor [Serafino et al., [2003]]; recipient cells were the human hepatoma HepG2 cell line. Both cell types were co-cultured for 48 hr to allow the cell-to-cell contacts. The hepatoma HepG2 cells are not permissive to free-virus infection, but they were infected successfully using TO.FEHCV cells as source of virus. The kinetics of viral RNA synthesis and the percentage of infected cells were compared in cell-mediated-and cell-free-viral infection. After co-cultivation, a consistent proportion of hepatoma cells replicated HCV and stably expressed viral antigens. Virus produced was infectious as demonstrated by the ability to reinfect fresh B-cells. This cell model shows that permissiveness to HCV infection can be achieved in vitro in non-permissive hepatoma cells by direct cell-to-cell contacts with infected human B-cells. This mechanism of virus spread may also play a pathogenic role in vivo. (literal)
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