Effects of gliadin stimulation on bone marrow-derived dendritic cells from HLA-DQ8 transgenic MICE (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Effects of gliadin stimulation on bone marrow-derived dendritic cells from HLA-DQ8 transgenic MICE (Articolo in rivista) (literal)

Anno

• 2008-01-01T00:00:00+01:00 (literal)

Alternative label

• Ciccocioppo R.; Rossi M.; Pesce I.; Ricci G.; Millimaggi D.; Maurano F.; Corazza G.R. (2008)
  Effects of gliadin stimulation on bone marrow-derived dendritic cells from HLA-DQ8 transgenic MICE
  in Digestive and Liver Disease
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Ciccocioppo R.; Rossi M.; Pesce I.; Ricci G.; Millimaggi D.; Maurano F.; Corazza G.R. (literal)

Pagina inizio

• 927 (literal)

Pagina fine

• 935 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 40 (literal)

Rivista

• Digestive and Liver Disease (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 12 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Istituto di scienza dell'alimentazione, CNR, Avellino, Italy Center for the Study and Cure of Coeliac Disease, IRCCS San Matteo Hospital Foundation, University of Pavia, Italy. (literal)

Titolo

• Effects of gliadin stimulation on bone marrow-derived dendritic cells from HLA-DQ8 transgenic MICE (literal)

Abstract

• BACKGROUND AND AIM: Gliadin presentation by HLA-DQ2/8 molecules to T cells plays a crucial role in triggering the inflammatory cascade in coeliac disease. We aimed to study the immunological effects of gliadin stimulation on dendritic cells (DCs) from HLA-DQ8 transgenic and BALB/c mice. METHODS: Bone marrow-derived DCs were stimulated with alpha-chymotrypsin-digested gliadin or ovoalbumin (100mug/ml). Modification of DC maturation, through HLA-DQ8 and MHC class II expression, and activation, by CD80 and CD86, was assessed by flow cytometry. The ability of pulsed and unpulsed DCs to prime T cells was evaluated by mixed leucocyte reaction. The expression of interleukin-4, -10, -12p70 and interferon-alpha, as well as of Toll-like receptor-4, -7, -8, -9 was determined by ELISA and real-time RT-PCR, respectively. RESULTS: Gliadin stimulation induced DC maturation (p<0.001 in BALB/c, p<0.01 in DQ8) but not activation, whereas ovoalbumin upregulated all markers (p<0.01 for maturation and p<0.001 for activation in both DC populations). No increase of T proliferation was elicited by pulsed DCs with respect to unpulsed DCs. Only in DQ8 DCs, gliadin induced Toll-like receptor-4 (p<0.001), -7 (p<0.001), -8 (p<0.005) expression and interferon-alpha (p<0.001) secretion. CONCLUSION: Gliadin resulted unable to activate DC, but stimulated Toll-like receptor expression and interferon-alpha secretion. (literal)

Prodotto di

• Tecnologie, Tracciabilità e Sicurezza degli Alimenti (AG.P05.004.001) (Modulo)
• Institute of food sciences (ISA) (Istituto)

Autore CNR

• MAURO ROSSI (Unità di personale interno)
• FRANCESCO MAURANO (Persona)

Incoming links:

Prodotto

• Institute of food sciences (ISA) (Istituto)
• Tecnologie, Tracciabilità e Sicurezza degli Alimenti (AG.P05.004.001) (Modulo)

Autore CNR di

• MAURO ROSSI (Unità di personale interno)
• FRANCESCO MAURANO (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Digestive and Liver Disease (Rivista)