Coupling Immunomagnetic Separation on Magnetic Beads with MALDI-TOF Mass Spectrometry for the Detection of Staphylococcal Enterotoxin B (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Coupling Immunomagnetic Separation on Magnetic Beads with MALDI-TOF Mass Spectrometry for the Detection of Staphylococcal Enterotoxin B (Articolo in rivista) (literal)

Anno

• 2007-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1128/AEM.01136-07 (literal)

Alternative label

• Schlosser Gitta; Kacer Petr; Kuzma Marek; Szilagyi Zoltan; Sorrentino Alida; Manzo Carla; Pizzano Rosa; Malorni Livia; Pocsfalvi Gabriella Katalin (2007)
  Coupling Immunomagnetic Separation on Magnetic Beads with MALDI-TOF Mass Spectrometry for the Detection of Staphylococcal Enterotoxin B
  in Applied and environmental microbiology (Print); American Society Of Microbiology (ASM), Washington (Stati Uniti d'America)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Schlosser Gitta; Kacer Petr; Kuzma Marek; Szilagyi Zoltan; Sorrentino Alida; Manzo Carla; Pizzano Rosa; Malorni Livia; Pocsfalvi Gabriella Katalin (literal)

Pagina inizio

• 6945 (literal)

Pagina fine

• 6952 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 73 (literal)

Rivista

• Applied and environmental microbiology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 8 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 21 (literal)

Note

• Google Scholar (literal)
• Scopu (literal)
• ISI Web of Science (WOS) (literal)
• PubMe (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Malorni Livia, Manzo Carla, Pizzano Rosa, Pocsfalvi Gabriella Katalin, Sorrentino Alida: Istituto di scienza dell'alimentazione; Kacer Petr: Department of Organic Technology - Institute of Chemical Technology, Prague, Czech Republic; Schlosser Gitta: Research Group of Peptide Chemistry, Department of Organic Chemistry - Hungarian Academy of Sciences, Eötvös L. University, Budapest, Hungary; Kuzma Marek: Institute of Microbiology - Academy of Sciences of the Czech Republic, Prague, Czech Republic; Szilagyi Zoltan: Research Institute for Viticulture and Enology, Kolyukteto, H-3300, Eger, Hungary (literal)

Titolo

• Coupling Immunomagnetic Separation on Magnetic Beads with MALDI-TOF Mass Spectrometry for the Detection of Staphylococcal Enterotoxin B (literal)

Abstract

• The growing importance of mass spectrometry for the identification and characterization of bacterial protein toxins is a consequence of the improved sensitivity and specificity of mass spectrometry-based techniques, especially when these techniques are combined with affinity methods. Here we describe a novel method based on the use of immunoaffinity capture and matrix-assisted laser desorption ionization-time of flight mass spectrometry for selective purification and detection of staphylococcal enterotoxin B (SEB). SEB is a potent bacterial protein toxin responsible for food poisoning, as well as a potential biological warfare agent. Unambiguous detection of SEB at low-nanogram levels in complex matrices is thus an important objective. In this work, an affinity molecular probe was prepared by immobilizing anti-SEB antibody on the surface of para-toluene-sulfonyl-functionalized monodisperse magnetic particles and used to selectively isolate SEB. Immobilization and affinity capture procedures were optimized to maximize the density of anti-SEB immunoglobulin G and the amount of captured SEB, respectively, on the surface of magnetic beads. SEB could be detected directly \"on beads\" by placing the molecular probe on the matrix-assisted laser desorption ionization target plate or, alternatively, \"off beads\" after its acidic elution. Application of this method to complex biological matrices was demonstrated by selective detection of SEB present in different matrices, such as cultivation media of Staphylococcus aureus strains and raw milk samples. (literal)

Editore

• American Society Of Microbiology (ASM) (Editore)

Prodotto di

• Institute of food sciences (ISA) (Istituto)
• Metodologie di Spettrometria di Massa, Proteomica, Metabolomica e Bioinformatica nelle Scienze dell'Alimentazione (AG.P05.003.001) (Modulo)
• Tecnologie, Tracciabilità e Sicurezza degli Alimenti (AG.P05.004.001) (Modulo)

Autore CNR

• GABRIELLA KATALIN POCSFALVI (Persona)
• ROSA PIZZANO (Persona)
• ALIDA SORRENTINO (Unità di personale interno)
• LIVIA MALORNI (Persona)

Insieme di parole chiave

• Keywords of "Coupling Immunomagnetic Separation on Magnetic Beads with MALDI-TOF Mass Spectrometry for the Detection of Staphylococcal Enterotoxin B" (Insieme di parole chiave)

Incoming links:

Prodotto

• Institute of food sciences (ISA) (Istituto)
• Metodologie di Spettrometria di Massa, Proteomica, Metabolomica e Bioinformatica nelle Scienze dell'Alimentazione (AG.P05.003.001) (Modulo)
• Tecnologie, Tracciabilità e Sicurezza degli Alimenti (AG.P05.004.001) (Modulo)

Autore CNR di

• ALIDA SORRENTINO (Unità di personale interno)
• GABRIELLA KATALIN POCSFALVI (Persona)
• ROSA PIZZANO (Persona)
• LIVIA MALORNI (Persona)

Editore di

• American Society Of Microbiology (ASM) (Editore)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Applied and environmental microbiology (Rivista)

Insieme di parole chiave di

• Keywords of "Coupling Immunomagnetic Separation on Magnetic Beads with MALDI-TOF Mass Spectrometry for the Detection of Staphylococcal Enterotoxin B" (Insieme di parole chiave)