Interleukin-10 expression by real-time PCR and homology modelling analysis in the European sea bass (Dicentrarchu Labrax L.). (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Interleukin-10 expression by real-time PCR and homology modelling analysis in the European sea bass (Dicentrarchu Labrax L.). (Articolo in rivista) (literal)

Anno

• 2007-01-01T00:00:00+01:00 (literal)

Alternative label

• Buonocore F, Randelli E, Bird S, Secombes CJ, Facchiano A, Costantini S, Scapigliati G. (2007)
  Interleukin-10 expression by real-time PCR and homology modelling analysis in the European sea bass (Dicentrarchu Labrax L.).
  in Aquaculture (Amst.)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Buonocore F, Randelli E, Bird S, Secombes CJ, Facchiano A, Costantini S, Scapigliati G. (literal)

Pagina inizio

• 512 (literal)

Pagina fine

• 522 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni

• 0 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 270 (literal)

Rivista

• Aquaculture (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Titolo

• Interleukin-10 expression by real-time PCR and homology modelling analysis in the European sea bass (Dicentrarchu Labrax L.). (literal)

Abstract

• In this paper we investigate the interleukin-10 (IL-10) expression and 3D modelling of the European sea bass (Dicentrarchus labrax L.). IL-10 is a regulatory cytokine that has been intensively studied in mammals and has been found mainly involved in the suppression or deactivation of activated immune responses. The full-length sea bass IL-10 cDNA consists of 1015 bp and is translated in a predicted molecule of 187 amino acids. A multiple alignment of this peptide with other known sequences showed the conservation of fundamental IL-10 family characteristics. Expression analysis by real-time PCR in control fish showed a high basal expression in the head kidney (HK), followed by gut and brain. In vitro treatment of HK leucocytes with LPS showed an intense increase of IL-10 expression at 4 h and a significant decrease at 24 h, with PHA-L no differences were evidenced. A 3D model for sea bass IL-10 was obtained by accurate homology procedures and putative interaction residues with the IL-10 receptor described. The results suggested that sea bass IL-10 could be involved in anti-inflammatory responses, but further experiments are needed to assess its importance in response to pathogenic agents, vaccinations and immunostimulants. (literal)

Prodotto di

• Institute of food sciences (ISA) (Istituto)
• Bioinformatica per lo studio di patologie umane, intolleranze alimentari e sicurezza alimentare (INT.P02.006.001) (Modulo)
• Metodologie di Spettrometria di Massa, Proteomica, Metabolomica e Bioinformatica nelle Scienze dell'Alimentazione (AG.P05.003.001) (Modulo)

Autore CNR

• ANGELO FACCHIANO (Unità di personale interno)
• SUSAN COSTANTINI (Unità di personale esterno)

Incoming links:

Prodotto

• Institute of food sciences (ISA) (Istituto)
• Bioinformatica per lo studio di patologie umane, intolleranze alimentari e sicurezza alimentare (INT.P02.006.001) (Modulo)
• Metodologie di Spettrometria di Massa, Proteomica, Metabolomica e Bioinformatica nelle Scienze dell'Alimentazione (AG.P05.003.001) (Modulo)

Autore CNR di

• ANGELO FACCHIANO (Unità di personale interno)
• SUSAN COSTANTINI (Unità di personale esterno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Aquaculture (Rivista)