MALDI-TOF mass spectrometry for the discrimination of foodborne microorganisms (Articolo in rivista)

Type
Label
  • MALDI-TOF mass spectrometry for the discrimination of foodborne microorganisms (Articolo in rivista) (literal)
Anno
  • 2006-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1128/AEM.72.2.1180-1189.2006 (literal)
Alternative label
  • Mazzeo MF; Sorrentino A; Gaita M; Cacace G; Di Stasio M; Facchiano A; Comi G; Malorni A and Siciliano RA. (2006)
    MALDI-TOF mass spectrometry for the discrimination of foodborne microorganisms
    in Applied and environmental microbiology (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Mazzeo MF; Sorrentino A; Gaita M; Cacace G; Di Stasio M; Facchiano A; Comi G; Malorni A and Siciliano RA. (literal)
Pagina inizio
  • 1631 (literal)
Pagina fine
  • 1638 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 72 (literal)
Rivista
Note
  • PubMe (literal)
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Proteomic and Biomolecular Mass Spectrometry Center, Institute of Food Science and Technology, CNR, Avellino, Italy, and Department of Food Science, University of Udine, Udine, Italy (literal)
Titolo
  • MALDI-TOF mass spectrometry for the discrimination of foodborne microorganisms (literal)
Abstract
  • A methodology based on matrix-assisted laser desorption ionization-time of flight mass spectrometry of intact bacterial cells was used for rapid discrimination of 24 bacterial species, and detailed analyses to identify Escherichia coli O157:H7 were carried out. Highly specific mass spectrometric profiles of pathogenic and nonpathogenic bacteria that are well-known major food contaminants were obtained, uploaded in a specific database, and made available on the Web. In order to standardize the analytical protocol, several experimental, sample preparation, and mass spectrometry parameters that can affect the reproducibility and accuracy of data were evaluated. Our results confirm the conclusion that this strategy is a powerful tool for rapid and accurate identification of bacterial species and that mass spectrometric methodologies could play an essential role in polyphasic approaches to the identification of pathogenic bacteria. (literal)
Prodotto di
Autore CNR

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