Butterfat fatty acids differentially regulate growth and differentiation in Jurkat T-cells. (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Butterfat fatty acids differentially regulate growth and differentiation in Jurkat T-cells. (Articolo in rivista) (literal)

Anno

• 2005-01-01T00:00:00+01:00 (literal)

Alternative label

• Bergamo P.; Luongo D.; Maurano F.; Rossi M. (2005)
  Butterfat fatty acids differentially regulate growth and differentiation in Jurkat T-cells.
  in Journal of cellular biochemistry (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Bergamo P.; Luongo D.; Maurano F.; Rossi M. (literal)

Pagina inizio

• 349 (literal)

Pagina fine

• 360 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 96 (literal)

Rivista

• Journal of cellular biochemistry (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Istituto di Scienze dell'Alimentazione, CNR, Avellino, Italy (literal)

Titolo

• Butterfat fatty acids differentially regulate growth and differentiation in Jurkat T-cells. (literal)

Abstract

• Synthetic Conjugated Linoleic Acid mixture (CLA; c9,t11; t10,c12-18:2) has been previously shown to inhibit growth, and enhance apoptosis and IL-2 mRNA synthesis in human lymphoblastic Jurkat T-cells. In this study, two different butterfat types were evaluated and compared for their effects on Jurkat cell viability, oxidative stress, pro-apoptotic activity, and cytokine synthesis: the conventionally produced butterfat (CBF), and organic butterfat (OBF) containing significantly higher amounts of c9,t11 (Rumenic Acid, RA), trans-vaccenic acid (VA; t11-18:1), alpha-linolenic acid (ALA), and lower levels of linoleic acid (LA). Results from cell treatment with both butterfat mixtures showed comparable oxidative stress (superoxide production, intracellular GSH depletion,and lipid peroxides yield), NADPH oxidase activation, cytotoxicity (LDH release), and IL-2 transcript level, whereas the effects of enhanced growth-inhibitory and pro-apoptotic activities were associated with OBF treatment. To then investigate each butterfat-induced effect caused by RA, VA, LA, and ALA, cells were exposed to synthetic FA concentrations similar to those from the different butterfats. Higher oxidative stress (superoxide production, intracellular GSH depletion) was induced by alpha-linolenic (ALA) and linoleic (LA) incubation (P<0.01) and superoxide production was suppressed by specific PKCalpha inhibitor (Go 6976) and linked to increased toxicity and IL-2 synthesis inhibition. By contrast, cell treatment with RA increased apoptosis and IL-2 synthesis. These results suggest that a supply of ALA and LA is responsible for BF-induced oxidative stress via PKCalpha-NADPH oxidase pathway, and that enhanced antiproliferative effects in OBF treated cells is essentially determined by RA-induced pro-apoptotic activity. Copyright (c) 2005 Wiley-Liss, Inc. (literal)

Prodotto di

• Tecnologie, Tracciabilità e Sicurezza degli Alimenti (AG.P05.004.001) (Modulo)
• Alimenti e salute dell'uomo (AG.P05.005.001) (Modulo)
• Institute of food sciences (ISA) (Istituto)

Autore CNR

• FRANCESCO MAURANO (Persona)
• PAOLO BERGAMO (Unità di personale interno)
• DIOMIRA LUONGO (Persona)
• MAURO ROSSI (Unità di personale interno)

Incoming links:

Prodotto

• Institute of food sciences (ISA) (Istituto)
• Tecnologie, Tracciabilità e Sicurezza degli Alimenti (AG.P05.004.001) (Modulo)
• Alimenti e salute dell'uomo (AG.P05.005.001) (Modulo)

Autore CNR di

• PAOLO BERGAMO (Unità di personale interno)
• MAURO ROSSI (Unità di personale interno)
• FRANCESCO MAURANO (Persona)
• DIOMIRA LUONGO (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of cellular biochemistry (Rivista)