http://www.cnr.it/ontology/cnr/individuo/prodotto/ID45588
Zonula occludens toxin (Zot) interferes with the induction of nasal tolerance to gliadin. (Articolo in rivista)
- Type
- Label
- Zonula occludens toxin (Zot) interferes with the induction of nasal tolerance to gliadin. (Articolo in rivista) (literal)
- Anno
- 2002-01-01T00:00:00+01:00 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Rossi M; Maurano F; Luongo D; Fasano A; Uzzau S; Auricchio S; Troncone R. (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Istituto di Scienze dell'Alimentazione, CNR, Via Roma 52, 83100 Avellino, Italy
Department of Physiology, Division of Pediatric Gastroenterology and Nutrition, Gastrointestinal Pathophysiology Section, Center for Vaccine
Development, University of Maryland School of Medicine, Baltimore, MD, USA
Department of Pediatrics, European Laboratory of Food Intolerances and Diseases, University 'Federico II', Naples, Italy (literal)
- Titolo
- Zonula occludens toxin (Zot) interferes with the induction of nasal tolerance to gliadin. (literal)
- Abstract
- Both nasal and oral administration of soluble protein antigens (Ags) induce tolerance, a phenomenon that has hampered mucosal vaccine design.
To produce active immunity the use of adjuvants co-administered with soluble Ags is required. Cholera toxin (CT) and Escherichia coli heat-
labile enterotoxin (LT) were found to be powerful mucosal adjuvants, but they are not suitable for clinical use because of their associated
toxicity. Therefore, there is the need to develop alternative strategies to deliver Ag in order to induce immunoprotection. Among these innovative
tools, a new toxin, Zonula occludens toxin (Zot), produced by phages in toxigenic strains of Vibrio cholerae, has been recently exploited for its
adjuvant activity at the mucosal level. The present study was undertaken to further highlight the adjuvant properties of Zot. The ability of Zot
to induce a mucosal response to gliadin was demonstrated per serum antibody production. In our established model of systemic tolerance to
gliadin, induced by its nasal administration, we found a reduced production of interferon-gamma (IFN-gamma) and interleukin-2 (IL-2) upon
administration of gliadin alone. This immune suppression was reverted in mice receiving gliadin together with Zot. As previously shown, the down-
regulation of Th1-like cytokines was found to be associated to a suppression of the T-cell proliferation, while such a suppression was
completely reverted by Zot co-administration. In conclusion, these data confirm Zot as a good mucosal adjuvant, considering its ability to
interfere with the suppression of specific cell mediated immunity, probably as a result of the increased dose and/or altered processing of
Ag at mucosal level. (literal)
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