http://www.cnr.it/ontology/cnr/individuo/prodotto/ID45585
Down-regulation of ERK1 and ERK2 activity during differentiation of the intestinal cell line HT-29. (Articolo in rivista)
- Type
- Label
- Down-regulation of ERK1 and ERK2 activity during differentiation of the intestinal cell line HT-29. (Articolo in rivista) (literal)
- Anno
- 2002-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1023/A:1014476706382 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Luongo D; Mazzarella G; Della Ragione F; Maurano F; Rossi M. (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Istituto di Scienze dell'Alimentazione, Consiglio Nazionale delle Ricerche, Avellino, Italy.
Second University of Naples, Medical School, Department of Biochemistry and Biophysics F. Cedrangolo, Naples, Italy. (literal)
- Titolo
- Down-regulation of ERK1 and ERK2 activity during differentiation of the intestinal cell line HT-29. (literal)
- Abstract
- The role and regulation of signal transduction pathways in proliferation
and differentiation of intestinal epithelial cells are still poorly
understood. However, growing evidences have been recently accumulated
demonstrating that mitogen-activated protein kinases (MAPKs) play a
pivotal function in the normal development of intestine. We have
investigated, in the intestinal cell line HT-29, the regulation (namely
activity and phosphorylation degree) of MAP kinases ERK 1 (p44) and ERK 2
(p42) during differentiation. Addition of fetal calf serum to HT-29
undifferentiated resting cells caused a rapid phosphorylation of both
ERKs and an increase of their specific kinase activity. Moreover, nuclear
translocation of ERK 1 and ERK 2 occurred concurrently to their
activation, leading to the conclusion that ERK 1 and ERK 2 are
classically regulated when quiescent HT-29 cells are induced to
proliferate. Butyrate addition to the intestinal cell line resulted in
terminal differentiation and in a selective down-regulation of ERK 2
activity (and phosphorylation degree) without any effect on ERK 1.
Conversely, when HT-29 cells were differentiated by repeated passages in
a glucose-free medium, we observed a progressive dephosphorylation and
inactivation of p42 and p44 kinases along with the failure of serum to
activate both the enzymes. Our findings suggest that, during the
differentiation of intestinal cells, remarkable changes occur in ERK 1
and ERK 2 control mechanisms leading to an unresponsiveness of MAP kinase
pathway. (literal)
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