Interaction of DAPI with individual strands of trinucleotide repeats. Effects on replication in vitro of the AAT. ATT triplet. (Articolo in rivista)

Type
Label
  • Interaction of DAPI with individual strands of trinucleotide repeats. Effects on replication in vitro of the AAT. ATT triplet. (Articolo in rivista) (literal)
Anno
  • 2003-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1046/j.1432-1033.2003.03877.x (literal)
Alternative label
  • Trotta E. 1, Del Grosso N. 2, Erba M. 3, Melino S. 4, Cicero D. 5, Paci M. 6 (2003)
    Interaction of DAPI with individual strands of trinucleotide repeats. Effects on replication in vitro of the AAT. ATT triplet.
    in European journal of biochemistry (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Trotta E. 1, Del Grosso N. 2, Erba M. 3, Melino S. 4, Cicero D. 5, Paci M. 6 (literal)
Pagina inizio
  • 4755 (literal)
Pagina fine
  • 4761 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni
  • Pubblicazione su rivista internazione con fattore di impatto (impact factor) pari a 3,0. (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 270 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto
  • Pubblicazione dei risultati sperimentali ottenuti dallo studio degli effetti indotti dal farmaco DAPI sulla struttura della tripletta AAT.ATT e sulla sua replicazione in vitro. (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1,2 INMM CNR, 3,4,5,6 UNI Roma Tor Vergata (literal)
Titolo
  • Interaction of DAPI with individual strands of trinucleotide repeats. Effects on replication in vitro of the AAT. ATT triplet. (literal)
Abstract
  • The structural changes produced by the minor-groove binding ligand DAPI (4',6-diamidine-2-phenylindole) on individual strands of trinucleotide repeat sequences were detected by electrophoretic band-shift analysis and related to their effects on DNA replication in vitro. Among the 20 possible single-stranded trinucleotide repeats, only the T-rich strand of the AAT.ATT triplet exhibits an observable fluorescence band and a change in electrophoretic mobility due to the drug binding. This is attributable to the property of DAPI that favours folding of the random coil ATT strand into a fast-migrating hairpin structure by a minor-groove binding mechanism. Electrophoretic characteristics of AAT, ACT, AGT, ATG and ATC are unchanged by DAPI, suggesting the crucial role of T.T with respect to A.A, C.C and G.G mismatch, in favouring the binding properties and the structural features of the ATT–DAPI complexes. Primer extension experiments, using the Klenow fragment of DNA polymerase I, demonstrate that such a selective structural change at ATT targets presents a marked property to stall DNA replication in vitro in comparison with the complementary AAT and a random GC-rich sequence. The results suggest a novel molecular mechanism of action of the DNA minor-groove binding ligand DAPI. (literal)
Prodotto di
Autore CNR
Insieme di parole chiave

Incoming links:


Autore CNR di
Prodotto
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi
Insieme di parole chiave di
data.CNR.it