http://www.cnr.it/ontology/cnr/individuo/prodotto/ID4553
Interaction of DAPI with individual strands of trinucleotide repeats. Effects on replication in vitro of the AAT. ATT triplet. (Articolo in rivista)
- Type
- Label
- Interaction of DAPI with individual strands of trinucleotide repeats. Effects on replication in vitro of the AAT. ATT triplet. (Articolo in rivista) (literal)
- Anno
- 2003-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1046/j.1432-1033.2003.03877.x (literal)
- Alternative label
Trotta E. 1, Del Grosso N. 2, Erba M. 3, Melino S. 4, Cicero D. 5, Paci M. 6 (2003)
Interaction of DAPI with individual strands of trinucleotide repeats. Effects on replication in vitro of the AAT. ATT triplet.
in European journal of biochemistry (Print)
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Trotta E. 1, Del Grosso N. 2, Erba M. 3, Melino S. 4, Cicero D. 5, Paci M. 6 (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni
- Pubblicazione su rivista internazione con fattore di impatto (impact factor) pari a 3,0. (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto
- Pubblicazione dei risultati sperimentali ottenuti dallo studio degli effetti indotti dal farmaco DAPI sulla struttura della tripletta AAT.ATT e sulla sua replicazione in vitro. (literal)
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- 1,2 INMM CNR,
3,4,5,6 UNI Roma Tor Vergata (literal)
- Titolo
- Interaction of DAPI with individual strands of trinucleotide repeats. Effects on replication in vitro of the AAT. ATT triplet. (literal)
- Abstract
- The structural changes produced by the minor-groove
binding ligand DAPI (4',6-diamidine-2-phenylindole) on
individual strands of trinucleotide repeat sequences were
detected by electrophoretic band-shift analysis and related to
their effects on DNA replication in vitro. Among the 20
possible single-stranded trinucleotide repeats, only the T-rich
strand of the AAT.ATT triplet exhibits an observable
fluorescence band and a change in electrophoretic mobility
due to the drug binding. This is attributable to the property
of DAPI that favours folding of the random coil ATT strand
into a fast-migrating hairpin structure by a minor-groove
binding mechanism. Electrophoretic characteristics of AAT,
ACT, AGT, ATG and ATC are unchanged by DAPI,
suggesting the crucial role of T.T with respect to A.A, C.C
and G.G mismatch, in favouring the binding properties and
the structural features of the ATTDAPI complexes. Primer
extension experiments, using the Klenow fragment of DNA
polymerase I, demonstrate that such a selective structural
change at ATT targets presents a marked property to stall
DNA replication in vitro in comparison with the complementary
AAT and a random GC-rich sequence. The results
suggest a novel molecular mechanism of action of the DNA
minor-groove binding ligand DAPI. (literal)
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