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Suggested role of the Golgi apparatus and endoplasmic reticulum as crucial sites of hepatitis C virus replication in human lymphoblastoid cells infected in vitro. (Articolo in rivista)

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Suggested role of the Golgi apparatus and endoplasmic reticulum as crucial sites of hepatitis C virus replication in human lymphoblastoid cells infected in vitro. (Articolo in rivista) (literal)

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Serafino A. 1, Valli M.B. 2, Andreola F. 3, Crema A. 4, Ravagnan G. 5, Bertolini L. 6, Carloni G. 7 (2003)
Suggested role of the Golgi apparatus and endoplasmic reticulum as crucial sites of hepatitis C virus replication in human lymphoblastoid cells infected in vitro.
in Journal of medical virology (Print)
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Serafino A. 1, Valli M.B. 2, Andreola F. 3, Crema A. 4, Ravagnan G. 5, Bertolini L. 6, Carloni G. 7 (literal)

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Impact factor: 2.881 I dati basati su tecniche di microscopia elettronica e confocale indicano che L'apparato del Golgi ed il reticolo endoplasmico giocano un ruolo di primo piano nel processo di assemblaggio e di morfogenesi del virus HCV. (literal)

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I risultati ottenuti mediante inoculazione in vitro con il virus HCV indicano che le cellule della linea lifoblastoide B umana TO.FE non solo sono suscettibili all'infezione ma che sono capaci di produrre in modo efficiente e duraturo particelle virali complete ed infettanti. Ciò fa di questo sistema cellulare un modello di scelta per elucidare il meccanismo d'infezione e di replicazione di tale virus. (literal)

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1,2,3,4,5,6,7 , Consiglio Nazionale delle Ricerche (CNR),INMM, Institute of Neurobiology and Molecular Medicine, Research Area of Tor Vergata, Rome, Italy (literal)

Titolo

Suggested role of the Golgi apparatus and endoplasmic reticulum as crucial sites of hepatitis C virus replication in human lymphoblastoid cells infected in vitro. (literal)

Abstract

Iacovacci et al. [(1997a) Research in Virology 148:147-151] described that the euploid diploid cells, of the normal human bone marrow-derived lymphoblastoid B-cell line TO.FE., are susceptible to hepatitis C virus (HCV) infection and support long term virus production. Transmission electron microscopy described some steps of HCV replication cycle in this in vitro infected cellular system [Serafino et al. (1997) Research in Virology 148:153-159]. In the present study, in order to identify the intracellular sites involved in HCV replication, the ultrastructural changes associated with infection in TO.FE. cells were correlated with the subcellular localisation of structural and nonstructural viral proteins. Transmission electron microscopy and confocal microscopy data indicate that these viral proteins appeared located in the Golgi apparatus and endoplasmic reticulum, suggesting an active involvement of these compartments in viral assembly and morphogenesis. Furthermore, transmission and scanning electron microscopic observations on cultures infected chronically support the hypothesis that these cellular compartments may serve as starting sites of the morphological changes associated to viral infection and replication, leading to cell-cell fusion, syncytia formation, and finally lysis of infected cells and virus release. (literal)

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