Comparison between structure and transfection efficiency: a study of DC-Chol-DOPE / DNA complexes (Articolo in rivista)

Type
Label
  • Comparison between structure and transfection efficiency: a study of DC-Chol-DOPE / DNA complexes (Articolo in rivista) (literal)
Anno
  • 2004-01-01T00:00:00+01:00 (literal)
Alternative label
  • Congiu A.1, Pozzi D.2, Esposito C.3, Castellano C.4, Mossa G.5 (2004)
    Comparison between structure and transfection efficiency: a study of DC-Chol-DOPE / DNA complexes
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Congiu A.1, Pozzi D.2, Esposito C.3, Castellano C.4, Mossa G.5 (literal)
Pagina inizio
  • 43 (literal)
Pagina fine
  • 48 (literal)
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  • Impact Factor = 1.325 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 36(1) (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1,2,4 = Dipartimento di Fisica and INFM Università ‘La Sapienza’,Rome, Italy; 3,5 = CNR, INMM,Rome, Italy. (literal)
Titolo
  • Comparison between structure and transfection efficiency: a study of DC-Chol-DOPE / DNA complexes (literal)
Abstract
  • The supramolecular structural nature of some cationic liposomes-DNA complexes, currently used as vehicles in non-viral gene delivery, has been elucidated by recent X-ray diffraction experiments. The relationship between the chemico-physical properties of these self-assembled structures and their transfection efficiency is extensively studied. Here we report a first comprehensive structural study by using energy dispersive X-ray diffraction, of the complex DC-Chol--DOPE/DNA (3beta[N-(N',N'-dimethylaminoethane)-carbamoyl]cholesterol dioleoylphosphatidylethanolamine/DNA), which has been classified as one of the most effective in in-vivo experiments. Our results show that DC-Chol--DOPE/DNA lipoplexes have a columnar inverted hexagonal structure, which is not influenced by the cationic liposome/DNA charge ratio. The transfection efficiency of C6 rat glioma cells by DC-Chol--DOPE/DNA lipoplexes and the toxicity of lipoplexes to cells are dramatically affected by cationic liposome/DNA weight ratio.It seems therefore that the lipoplex structures have not any influence on transfection efficiency and toxicity in our experimental system. (literal)
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