Comparison between structure and transfection efficiency: a study of DC-Chol-DOPE / DNA complexes (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Comparison between structure and transfection efficiency: a study of DC-Chol-DOPE / DNA complexes (Articolo in rivista) (literal)

Anno

• 2004-01-01T00:00:00+01:00 (literal)

Alternative label

• Congiu A.1, Pozzi D.2, Esposito C.3, Castellano C.4, Mossa G.5 (2004)
  Comparison between structure and transfection efficiency: a study of DC-Chol-DOPE / DNA complexes
  
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Congiu A.1, Pozzi D.2, Esposito C.3, Castellano C.4, Mossa G.5 (literal)

Pagina inizio

• 43 (literal)

Pagina fine

• 48 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni

• Impact Factor = 1.325 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 36(1) (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1,2,4 = Dipartimento di Fisica and INFM Università ‘La Sapienza’,Rome, Italy; 3,5 = CNR, INMM,Rome, Italy. (literal)

Titolo

• Comparison between structure and transfection efficiency: a study of DC-Chol-DOPE / DNA complexes (literal)

Abstract

• The supramolecular structural nature of some cationic liposomes-DNA complexes, currently used as vehicles in non-viral gene delivery, has been elucidated by recent X-ray diffraction experiments. The relationship between the chemico-physical properties of these self-assembled structures and their transfection efficiency is extensively studied. Here we report a first comprehensive structural study by using energy dispersive X-ray diffraction, of the complex DC-Chol--DOPE/DNA (3beta[N-(N',N'-dimethylaminoethane)-carbamoyl]cholesterol dioleoylphosphatidylethanolamine/DNA), which has been classified as one of the most effective in in-vivo experiments. Our results show that DC-Chol--DOPE/DNA lipoplexes have a columnar inverted hexagonal structure, which is not influenced by the cationic liposome/DNA charge ratio. The transfection efficiency of C6 rat glioma cells by DC-Chol--DOPE/DNA lipoplexes and the toxicity of lipoplexes to cells are dramatically affected by cationic liposome/DNA weight ratio.It seems therefore that the lipoplex structures have not any influence on transfection efficiency and toxicity in our experimental system. (literal)

Prodotto di

• Istituto di neurobiologia e medicina molecolare (INMM) (Istituto)

Autore CNR

• CLAUDIO ESPOSITO (Unità di personale interno)
• GIUSEPPE MOSSA (Unità di personale interno)

Insieme di parole chiave

• Parole chiave di "Comparison between structure and transfection efficiency: a study of DC-Chol-DOPE / DNA complexes" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• CLAUDIO ESPOSITO (Unità di personale interno)
• GIUSEPPE MOSSA (Unità di personale interno)

Prodotto

• Istituto di neurobiologia e medicina molecolare (INMM) (Istituto)

Insieme di parole chiave di

• Parole chiave di "Comparison between structure and transfection efficiency: a study of DC-Chol-DOPE / DNA complexes" (Insieme di parole chiave)