Effects of 2.45 GHz Microwave Fields on Liposomes Entrapping Glycoenzyme Ascorbate Oxidase: Evidence for Oligosaccharide Side Chain Involvement (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Effects of 2.45 GHz Microwave Fields on Liposomes Entrapping Glycoenzyme Ascorbate Oxidase: Evidence for Oligosaccharide Side Chain Involvement (Articolo in rivista) (literal)

Anno

• 2004-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1002/bem.10203 (literal)

Alternative label

• Ramundo-Orlando A.1; Liberti M.2; Mossa G.1; D'Inzeo G.2 (2004)
  Effects of 2.45 GHz Microwave Fields on Liposomes Entrapping Glycoenzyme Ascorbate Oxidase: Evidence for Oligosaccharide Side Chain Involvement
  in Bioelectromagnetics
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Ramundo-Orlando A.1; Liberti M.2; Mossa G.1; D'Inzeo G.2 (literal)

Pagina inizio

• 338 (literal)

Pagina fine

• 345 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni

• Impact Factor 1.243 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 25 (literal)

Rivista

• Bioelectromagnetics (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 5 (literal)

Note

• PubMe (literal)
• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1 = ICEmB at CNR INMM , Rome, Italy; 2 = ICEmB at Department of Electronic Engineering, University \"La Sapienza\", Rome, Italy (literal)

Titolo

• Effects of 2.45 GHz Microwave Fields on Liposomes Entrapping Glycoenzyme Ascorbate Oxidase: Evidence for Oligosaccharide Side Chain Involvement (literal)

Abstract

• Previous observations reported by our group indicate that 2.45 GHz microwave fields at specific absorption rate (SAR) of 5.6 W/kg reduce the enzyme activity rate of ascorbate oxidase (AO) trapped in liposomes.In this study, we report dose-response studies on these AO containing liposomes irradiated at different SAR values (1.4, 2.8, 4.2 and 5.6 W/kg. No response was observed for SAR below 5.6 W/kg. Liposomes entrapping functional AO in its deglycated form (AO-D) were also used. In this case, no MW related enzyme activity changes were observed, demonstrating a direct involvement of oligosaccharide chains of AO. Furthermore, the catalytic properties of both AO and AO-D were not impaired bu MW irradiation, neither in homogeneous solution nor loaded in liposomes, excluding possible changes in the conformation of enzyme as a mechanism. Our results suggest that the oligosaccharide chains of AO are critical to elicit the microwave observed effects on lipid membrane. (literal)

Prodotto di

• Studio dell'interazione dei campi elettromagnetici con modelli di membrana cellulare (ME.P06.012.006) (Modulo)
• Istituto di neurobiologia e medicina molecolare (INMM) (Istituto)

Autore CNR

• ALFONSINA RAMUNDO ORLANDO (Persona)

Insieme di parole chiave

• Parole chiave di "Effects of 2.45 GHz Microwave Fields on Liposomes Entrapping Glycoenzyme Ascorbate Oxidase: Evidence for Oligosaccharide Side Chain Involvement" (Insieme di parole chiave)

Incoming links:

Prodotto

• Istituto di neurobiologia e medicina molecolare (INMM) (Istituto)
• Studio dell'interazione dei campi elettromagnetici con modelli di membrana cellulare (ME.P06.012.006) (Modulo)

Autore CNR di

• ALFONSINA RAMUNDO ORLANDO (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Bioelectromagnetics (Rivista)

Insieme di parole chiave di

• Parole chiave di "Effects of 2.45 GHz Microwave Fields on Liposomes Entrapping Glycoenzyme Ascorbate Oxidase: Evidence for Oligosaccharide Side Chain Involvement" (Insieme di parole chiave)