Effect of P2 purinoceptor antagonists on kainate-induced currents in rat cultured neurons. (Articolo in rivista)

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  • Effect of P2 purinoceptor antagonists on kainate-induced currents in rat cultured neurons. (Articolo in rivista) (literal)
Anno
  • 2000-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/S0006-8993(00)02781-5 (literal)
Alternative label
  • Zona C., Marchetti C., Volonté C., Mercuri N., Bernardi G. (2000)
    Effect of P2 purinoceptor antagonists on kainate-induced currents in rat cultured neurons.
    in Brain research
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Zona C., Marchetti C., Volonté C., Mercuri N., Bernardi G. (literal)
Pagina inizio
  • 26 (literal)
Pagina fine
  • 35 (literal)
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  • IF = 2.626 SCI-JCR 2000 (literal)
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  • 882 (literal)
Rivista
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  • 1-2 (literal)
Note
  • ISI Web of Science (WOS) (literal)
  • PubMe (literal)
  • Scopu (literal)
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  • Università Tor Vergata, CNR-INMM, Italy (literal)
Titolo
  • Effect of P2 purinoceptor antagonists on kainate-induced currents in rat cultured neurons. (literal)
Abstract
  • The action of purinergic antagonists on kainate-induced currents was studied in rat cortical neurons in primary culture using the whole-cell configuration of the patch-clamp technique. The amplitude of the currents induced by kainate in cortical neurons was concentration-dependent (EC(50)=106 microM). Pyridoxal-phosphate-6-azophenyll-2',4'-disulphonic acid 4-sodium (PPADS), a P2X antagonist, was ineffective in the reduction of the kainate-induced current in cortical neurons, while 2, 2'-pyridylisatogen (PIT), basilen blue (BB) and suramin, respectively two selective P2Y and a non-selective P2 receptor antagonist, caused a reduction in the amplitude of the current induced by kainate. BB decreased the inward current induced by kainate at all holding potentials and the reduction was dose-dependent (EC(50)=34 microM). The total conductance of the neurons for the kainate-induced current was significantly reduced (P<0.01) and the effect was completely reversible. BB furthermore reduced the kainate-induced current in granule and hippocampal neurons and decreased the amplitude of the alpha-amino-3-hydroxy-5-methyl-4-isoxalepropionic acid (AMPA)-evoked current in cortical neurons. Cholera toxin (ChTx) did not affect the action of BB on the kainate-induced currents in cortical neurons and moreover, when guanosine 5'-o-(3-thiotriphosphate) (GTPgammaS) was added to the electrode solution, the kainate-induced currents were still reduced by 100 microM BB. The maximal response to kainate decreased in the presence of 20 microM BB without changing its EC(50), indicating a non-competitive mechanism of inhibition. These results demonstrate that preferential P2Y receptor antagonists are able to modulate the kainate and AMPA-induced currents in central neurons, suggesting a potential use of these compounds as neuroprotective agents. (literal)
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