Modification to the Lampariello approach to evaluate reactive oxygen species production by flow cytometry (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Modification to the Lampariello approach to evaluate reactive oxygen species production by flow cytometry (Articolo in rivista) (literal)

Anno

• 2008-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1002/cyto.a.20508 (literal)

Alternative label

• Brescia F; Sarti M; (2008)
  Modification to the Lampariello approach to evaluate reactive oxygen species production by flow cytometry
  in Cytometry. Part A (Online)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Brescia F; Sarti M; (literal)

Pagina inizio

• 175 (literal)

Pagina fine

• 179 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

• http://onlinelibrary.wiley.com/doi/10.1002/cyto.a.20508/abstract (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 73 (literal)

Rivista

• Cytometry. Part A (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• CNR-Istituto per il rilevamento elettromagnetico dell'ambiente CNR-Istituto per il rilevamento elettromagnetico dell'ambiente (literal)

Titolo

• Modification to the Lampariello approach to evaluate reactive oxygen species production by flow cytometry (literal)

Abstract

• The aim of this article is to perform a statistical analysis of reactive oxygen species (ROS) cytometric data. It is demonstrated that the classical parametric and nonparametric statistical tests are not suitable to examine these data; the Kolmogorov-Smirnov test and the modification proposed by Lampariello are shown to be too sensitive with respect to the experimental bias (due to procedure or to the instrument) and variability in the ROS production within the repeated samples. Several approaches are examined and discussed. Modifications of the Lampariello's procedure are proposed to include the variability within samples. The validity of the proposed approach is verified by analyzing repeated measurements of ROS formation in cultured human lymphocytes untreated or treated with ferrous sulfate. The proposed approach is successful in considering the \"intersample\" variability in the ROS data analysis and keeps a good level of validity. Nevertheless, this procedure is not user-friendly and needs to be handled by an expert operator. (literal)

Prodotto di

• effetti biologici dei campi elettromagnetici (INT.P01.006.002) (Modulo)
• Istituto per il rilevamento elettromagnetico dell'ambiente (IREA) (Istituto)

Autore CNR

• FRANCESCA BRESCIA (Persona)
• MAURIZIO SARTI (Unità di personale interno)

Insieme di parole chiave

• Parole chiave di "Modification to the Lampariello approach to evaluate reactive oxygen species production by flow cytometry" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• MAURIZIO SARTI (Unità di personale interno)
• FRANCESCA BRESCIA (Persona)

Prodotto

• Istituto per il rilevamento elettromagnetico dell'ambiente (IREA) (Istituto)
• effetti biologici dei campi elettromagnetici (INT.P01.006.002) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Cytometry. Part A (Rivista)

Insieme di parole chiave di

• Parole chiave di "Modification to the Lampariello approach to evaluate reactive oxygen species production by flow cytometry" (Insieme di parole chiave)