Surface properties of cholesterol-containing membranes detected by Prodan fluorescence. (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Surface properties of cholesterol-containing membranes detected by Prodan fluorescence. (Articolo in rivista) (literal)

Anno

• 2001-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/S0005-2736(01)00286-3 (literal)

Alternative label

• Krasnowska E.K., Bagatolli L.A., Gratton E., Parasassi T. (2001)
  Surface properties of cholesterol-containing membranes detected by Prodan fluorescence.
  in Biochimica et biophysica acta. Biomembranes
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Krasnowska E.K., Bagatolli L.A., Gratton E., Parasassi T. (literal)

Pagina inizio

• 330 (literal)

Pagina fine

• 340 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 1511 (literal)

Rivista

• Biochimica et biophysica acta. Biomembranes (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Istituto di Farmacologia Traslazionale, CNR, Roma Laboratory for Fluorescence Dynamics, University of Illinois at Urbana-Champaign, USA (literal)

Titolo

• Surface properties of cholesterol-containing membranes detected by Prodan fluorescence. (literal)

Abstract

• The fluorescent membrane probe 6-propionyl-2-dimethylaminonaphthalene (Prodan) displays a high sensitivity to the polarity and packing properties of lipid membrane. Contrary to 6-lauroyl-2-dimethylaminonaphthalene (Laurdan), Prodan can also monitor the properties of the membrane surface, i.e., the polar-head pretransition. In bilayers composed of coexisting gel and liquid^crystalline phases, Prodan shows a preferential partitioning in the latter, so that the detected membrane properties mainly belong to fluid domains. In the presence of cholesterol, the packing properties of the gel phase phospholipids are modified in such a way that Prodan can penetrate and label the membrane. Although Prodan labeling of the gel phase is a function of cholesterol concentration, 3 mol percent cholesterol is sufficient for a 60% Prodan labeling with respect to the maximum labeling reached at 15 mol percent cholesterol. We present steady-state and dynamical fluorescence measurements of Prodan in bilayers in the presence of cholesterol. Our results fit the liquid-ordered/liquid-disordered phase model for cholesterol-containing membranes and show that the presence of cholesterol, in addition to modification to the phase state of the hydrophobic portion of the bilayer, strongly affects the packing and the polarity of the membrane hydrophobic^hydrophilic interface. (literal)

Prodotto di

• Istituto di neurobiologia e medicina molecolare (INMM) (Istituto)
• Meccanismi ossidoriduttivi nelle patologie degenerative ed infiammatorie (ME.P04.009.003) (Modulo)

Autore CNR

• TIZIANA PARASASSI (Persona)
• EWA KRYSTYNA KRASNOWSKA (Unità di personale interno)

Incoming links:

Autore CNR di

• TIZIANA PARASASSI (Persona)
• EWA KRYSTYNA KRASNOWSKA (Unità di personale interno)

Prodotto

• Istituto di neurobiologia e medicina molecolare (INMM) (Istituto)
• Meccanismi ossidoriduttivi nelle patologie degenerative ed infiammatorie (ME.P04.009.003) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Biochimica et biophysica acta. Biomembranes (Rivista)