A NEW FLUORESCENT OLIGONUCLEOTIDE PROBE FOR IN SITU DETECTION OF S-TRIAZINE-DEGRADING RHODOCOCCUS WRATISLAVIENSIS IN CONTAMINATED GROUNDWATER AND SOIL SAMPLES (Articolo in rivista)

Type
Label
  • A NEW FLUORESCENT OLIGONUCLEOTIDE PROBE FOR IN SITU DETECTION OF S-TRIAZINE-DEGRADING RHODOCOCCUS WRATISLAVIENSIS IN CONTAMINATED GROUNDWATER AND SOIL SAMPLES (Articolo in rivista) (literal)
Anno
  • 2009-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.watres.2009.04.022 (literal)
Alternative label
  • GRENNI P. (a); GIBELLO A. (b); BARRA CARACCIOLO A. (a); FAJARDO C. (b); NANDE M. (b); SACCA' M.L. (a); MARTINEZ-INIGO M.J. (c); CICCOLI R. (d); MARTIN M. (b) (2009)
    A NEW FLUORESCENT OLIGONUCLEOTIDE PROBE FOR IN SITU DETECTION OF S-TRIAZINE-DEGRADING RHODOCOCCUS WRATISLAVIENSIS IN CONTAMINATED GROUNDWATER AND SOIL SAMPLES
    in Water research (Oxf.)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • GRENNI P. (a); GIBELLO A. (b); BARRA CARACCIOLO A. (a); FAJARDO C. (b); NANDE M. (b); SACCA' M.L. (a); MARTINEZ-INIGO M.J. (c); CICCOLI R. (d); MARTIN M. (b) (literal)
Pagina inizio
  • 2999 (literal)
Pagina fine
  • 3008 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
  • http://www.ncbi.nlm.nih.gov/pubmed/19476963 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 43 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 10 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 12 (literal)
Note
  • PubMe (literal)
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • a) Water Research Institute - National Research Council, Area della Ricerca di Roma 1-Montelibretti, via Salaria km 29,300, CP10 00016 Monterotondo, Rome, Italy; b) Facultad de Veterinaria, Complutense University Avenida Puerta de Hierro s/n, 28040 Madrid, Spain; c) Instituto Madrileno de Investigacion y Desarrollo Rural, Agrario y Alimentario (IMIDRA), Finca ''El Encin'' Km 38,2 A-II Apdo 127, 28800 Madrid, Spain; d) ENEA Centro Ricerche Casaccia Via Anguillarese, Rome, Italy (literal)
Titolo
  • A NEW FLUORESCENT OLIGONUCLEOTIDE PROBE FOR IN SITU DETECTION OF S-TRIAZINE-DEGRADING RHODOCOCCUS WRATISLAVIENSIS IN CONTAMINATED GROUNDWATER AND SOIL SAMPLES (literal)
Abstract
  • A bacterial strain (FPA1) capable of using terbuthylazine, simazine, atrazine, 2-hydroxysimazine, deethylatrazine, isopropylamine or ethylamine as its sole carbon source was isolated from a shallow aquifer chronically contaminated with s-triazine herbicides. Based on its 16S rDNA sequence analysis, the strain FPA1 was identified as Rhodococcus wratislaviensis. The disappearance time of 50% of the initial terbuthylazine concentration in the presence of this strain (DT50) was 62 days. This strain was also able to mineralise the [Uring 14C] triazine-ring, albeit at a slow rate. A 16S rRNA target oligonucleotide probe (RhLu) was designed, and the FISH protocol was optimised, in order to detect R. wratislaviensis in striazine- contaminated sites. The RhLu probe gave a positive signal (expressed as % of total DAPI-positive cells) in both the groundwater (2.19 ±0.41%) and soil (2.10 ±0.96%) samples analysed. Using the RhLu probe, R. wratislaviensis can be readily detected, and its population dynamics can be easily monitored, in soil and in water ecosystems contaminated with s-triazine. To the best of our knowledge, this is the first report showing the isolation, from groundwater, of a bacterial strain able to degrade s-triazines. (literal)
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