http://www.cnr.it/ontology/cnr/individuo/prodotto/ID42293
ESTROGENICITY PROFILE AND ESTROGENIC COMPOUNDS DETERMINED IN RIVER SEDIMENTS BY CHEMICAL ANALYSIS, ELISA AND YEAST ASSAYS (Articolo in rivista)
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- ESTROGENICITY PROFILE AND ESTROGENIC COMPOUNDS DETERMINED IN RIVER SEDIMENTS BY CHEMICAL ANALYSIS, ELISA AND YEAST ASSAYS (Articolo in rivista) (literal)
- Anno
- 2008-01-01T00:00:00+01:00 (literal)
- Alternative label
VIGANÒ L., BENFENATI E., CAUWENBERGE A., EIDEM J., ERRATICO C., GOKSOYR A., KLOAS W., MAGGIONI S., MANDICH A., URBATZKA R. (2008)
ESTROGENICITY PROFILE AND ESTROGENIC COMPOUNDS DETERMINED IN RIVER SEDIMENTS BY CHEMICAL ANALYSIS, ELISA AND YEAST ASSAYS
in Chemosphere
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- VIGANÒ L., BENFENATI E., CAUWENBERGE A., EIDEM J., ERRATICO C., GOKSOYR A., KLOAS W., MAGGIONI S., MANDICH A., URBATZKA R. (literal)
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- ISI Web of Science (WOS) (literal)
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- Water Research Institute, National Council of Research, Brugherio (Milan), Italy
Mario Negri Institute, Laboratory of Environmental Chemistry and Toxicology, Milan, Italy
Institut Provincial d'Hygiéne et de Bactériologie, Mons, Belgium
Biosense Laboratories AS, Bergen, Norway Department of Molecular Biology, University of Bergen, Bergen, Norway
Department of Inland Fisheries, Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Berlin, Germany
Department of Endocrinology, Institute of Biology, Humboldt-University, Berlin, Germany
Department of Environmental, Experimental and Applied Biology, University of Genoa, Genoa, Italy (literal)
- Titolo
- ESTROGENICITY PROFILE AND ESTROGENIC COMPOUNDS DETERMINED IN RIVER SEDIMENTS BY CHEMICAL ANALYSIS, ELISA AND YEAST ASSAYS (literal)
- Abstract
- An effects-directed strategy was applied to bed sediments of a polluted tributary in order to isolate and
identify the major estrogenic chemicals it discharges into the River Po, the principal Italian watercourse.
Sediment extract was concentrated by solid phase extraction and then fractioned into 10 fractions by
reversed phase high performance liquid chromatography (RP-HPLC). Estrogenic activity of whole extract
and fractions were determined using a recombinant yeast assay containing the human estrogen receptor
(YES). The 10 fractions and whole extract were analysed for target compounds, e.g. estrone (E1), 17bestradiol
(E2), estriol (E3), 4-nonylphenol (NP), 4-tert-octylphenol (t-OP), bisphenol A (BPA), using both
liquid chromatography-tandem mass spectrometry (LC-MS/MS) and non-competitive enzyme-linked
immunosorbent assays (ELISA). The YES assay determined high estrogenic activity in whole sediment
(15.6 ng/g EE2 equivalents), and positive results for fractions nr 1, 2, 6, 7 and 8. E1, E3 and NP were
the main estrogenic chemicals, however, other unidentified compounds contributed to sediment estrogenicity,
particularly for polar fractions nr 1 and 2. A GC-MS screening performed in scan mode identified
other potential contributors such as phthalates (DBP, BBP), and OP isomers. A next sampling campaign
extended to other tributaries and receiving stretches of the River Po confirmed E1, E3 and NP as major
estrogenic chemicals potentially threatening other sites of the main river. In general, target compound
ELISAs have been shown to be suitable tools for a rapid screening of wide areas or large numbers of environmental
samples for estrogenic risk. The potential for interferences suggests however to use cautiously
the concentration values obtained from some of the immunoassays. (literal)
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