http://www.cnr.it/ontology/cnr/individuo/prodotto/ID42141
APPLICATION OF FLUORESCENCE IN SITU HYBRIDIZATION TECHNIQUE TO DETECT SIMAZINE DEGRADING BACTERIA IN SOIL SAMPLES (Articolo in rivista)
- Type
- Label
- APPLICATION OF FLUORESCENCE IN SITU HYBRIDIZATION TECHNIQUE TO DETECT SIMAZINE DEGRADING BACTERIA IN SOIL SAMPLES (Articolo in rivista) (literal)
- Anno
- 2008-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1016/j.chemosphere.2007.10.071 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- MARTIN M. (a); GIBELLO A. (a); LOBO C. (b); NANDE M.(a); GARBI C.(a); FAJARDO C. (a); BARRA CARACCIOLO A. (c); GRENNI P. (c); MARTINEZ INIGO M.J. (b) (literal)
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- Rivista
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note
- (AVAILABLE ON LINE DOI:10.1016/J.CHEMOSPHERE 2007.10.071)
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- a) Complutense University, Avenida Puerta de Hierro s/n, 28040 Madrid, Spain
b) Instituto Madrileno de Investigacion y Desarrollo Rural, Agrario y Alimentario (IMIDRA),Finca ''El Enc?n \"Km 38,2 A-II Apdo 127, 28800 Madrid, Spain
c) Water Research Institute, National Research Council Via Reno, 1 00198 Rome, Italy (literal)
- Titolo
- APPLICATION OF FLUORESCENCE IN SITU HYBRIDIZATION TECHNIQUE TO DETECT SIMAZINE DEGRADING BACTERIA IN SOIL SAMPLES (literal)
- Abstract
- We propose a new approach to evaluate the natural attenuation capacity of soil by using fluorescence in situ hybridization (FISH). A specific oligonucleotide probe AtzB1 was designed based on the sequence data of the atzB gene involved in the hydrolytic deamination of s-triazines; this gene, located in a multiple copy plasmid was detected by the optimized FISH protocol. Two agricultural soils (Lodi and Henares) with a history of simazine treatments, and two natural soils (Soto and Monza), without previous exposure to simazine, were studied. AtzB1 probe-target cells were found only in the agricultural soils and, in a greater percentage, in the Lodi soil, compared to the Henares one. Moreover, the greatest percentage of AtzB1 probe-target cells in Lodi was accompanied by a greater mineralization rate, compared to the Henares soil. The FISH method used in this study was suitable for the detection of simazine-degrading bacteria and could be a useful indicator of the potential of soil bioremediation. (literal)
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