http://www.cnr.it/ontology/cnr/individuo/prodotto/ID4151
Cis- and trans-splicing of mRNAs mediated by tRNA sequences in eukaryotic cells (Articolo in rivista)
- Type
- Label
- Cis- and trans-splicing of mRNAs mediated by tRNA sequences in eukaryotic cells (Articolo in rivista) (literal)
- Anno
- 2008-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1073/pnas.0800420105 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Di Segni G., Gastaldi S. and Tocchini-Valentini G.P. (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#altreInformazioni
- Accepted Dec. 2007. Il lavoro descrive la scoperta di un nuovo modo di produrre RNA messaggeri chimerici (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
- http://www.pnas.org (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note
- Commented in: Anderson AM, Staley JP. Proc Natl Acad Sci U S A. 2008 105:6793-4 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto
- Pubblicazione su rivista internazionale (literal)
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Istituto di Biologia Cellulare (IBC)-CNR (literal)
- Titolo
- Cis- and trans-splicing of mRNAs mediated by tRNA sequences in eukaryotic cells (literal)
- Abstract
- The formation of chimeric mRNAs is a strategy used by human cells
to increase the complexity of their proteome, as revealed by the
ENCODE project. Here, we use Saccharomyces cerevisiae to show a
way by which trans-spliced mRNAs can be generated. We demonstrate
that a pretRNA inserted into a premRNA context directs the
splicing reaction precisely to the sites of the tRNA intron. A
suppressor pretRNA gene was inserted, in cis, into the sequence
encoding the third cytoplasmic loop of the Ste2 or Ste3 G proteincoupled
receptor. The hybrid RNAs are spliced at the specific
pretRNA splicing sites, releasing both functional tRNAs that suppress
nonsense mutations and translatable mRNAs that activate
the signal transduction pathway. The RNA molecules extracted
from yeast cells were amplified by RT-PCR, and their sequences
were determined, confirming the identity of the splice junctions.
We then constructed two fusions between the premRNA sequence
(STE2 or STE3) and the 5 - or 3 -pretRNA half, so that the two hybrid
RNAs can associate with each other, in trans, through their tRNA
halves. Splicing occurs at the predicted pretRNA sites, producing a
chimeric STE3-STE2 receptor mRNA. RNA trans-splicing mediated
by tRNA sequences, therefore, is a mechanism capable of producing
new kinds of RNAs, which could code for novel proteins. (literal)
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