http://www.cnr.it/ontology/cnr/individuo/prodotto/ID41248
Differential location of alpha-expansin proteins during the accommodation of root cells to an arbuscular mycorrhizal fungus. (Articolo in rivista)
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- Label
- Differential location of alpha-expansin proteins during the accommodation of root cells to an arbuscular mycorrhizal fungus. (Articolo in rivista) (literal)
- Anno
- 2005-01-01T00:00:00+01:00 (literal)
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- Balestrini R., Cosgrove D.J., Bonfante P. (literal)
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- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- -Balestrini R., Istituto per la Protezione delle Piante, CNR, Viale Mattioli 25, I-10125 Turin, Italy
-Cosgrove D.J., Pennsylvania State University, Department of Biology, University Pk, PA 16802 USA
-Bonfante P., Dipartimento di Biologia Vegetale, Università degli Studi di Torino, Viale Mattioli 25, I-10125 Turin, Italy (literal)
- Titolo
- Differential location of alpha-expansin proteins during the accommodation of root cells to an arbuscular mycorrhizal fungus. (literal)
- Abstract
- alpha-Expansins are extracellular proteins that increase plant cell-wall extensibility. We analysed their pattern of expression in cucumber roots in the presence and in the absence of the mycorrhizal fungus, Glomus versiforme. The distribution of alpha-expansins was investigated by use of two polyclonal antibodies (anti-EXPA1 and anti-EXPA2, prepared against two different cucumber alpha-expansins) in immunoblotting, immunofluorescence, and immunogold experiments. Immunoblot results indicate the presence of a 30-kDa band specific for mycorrhizal roots. The two antibodies identify antigens with a different distribution in mycorrhizal roots: anti-EXPA1 labels the interface zone, but the plant cell walls only weakly. By contrast, the anti-EXPA2 labels only the plant cell walls. In order to understand the potential role of alpha-expansins during the accommodation of the fungus inside root cells, we prepared semi-thin sections to measure the size of cortical cells and the thickness of cortical cell walls in mycorrhizal and non-mycorrhizal root. Mycorrhizal cortical cells were significantly larger than non-mycorrhizal cells and had thicker cell walls. In double-labelling experiments with cellobiohydrolase-gold complex, we observed that cellulose was co-localized with alpha-expansins. Taken together, the results demonstrate that alpha-expansins are more abundant in the cucumber cell walls upon mycorrhizal infection; we propose that these wall-loosening proteins are directly involved in the accommodation of the fungus by infected cortical cells. (literal)
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