Establishment of single-genotype axenic cultures from the haploid stage of the pine blister rust Cronartium flaccidum. (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Establishment of single-genotype axenic cultures from the haploid stage of the pine blister rust Cronartium flaccidum. (Articolo in rivista) (literal)

Anno

• 2001-01-01T00:00:00+01:00 (literal)

Alternative label

• Moricca S., Ragazzi A. (2001)
  Establishment of single-genotype axenic cultures from the haploid stage of the pine blister rust Cronartium flaccidum.
  in Mycological research (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Moricca S., Ragazzi A. (literal)

Pagina inizio

• 1527 (literal)

Pagina fine

• 1532 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 105 (literal)

Rivista

• Mycological research (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• -Moricca S., Istituto per la Protezione delle Piante, CNR, Sez. di Firenze, Via Madonna del Piano 10, I-50019 Sesto Fiorentino, Firenze, Italy -Ragazzi A., Dipartimento di Biotecnologie agrarie, Sez. Patologia vegetale, Università degli Studi di Firenze, Piazzale delle Cascine 28, I-50144 Firenze, Italy (literal)

Titolo

• Establishment of single-genotype axenic cultures from the haploid stage of the pine blister rust Cronartium flaccidum. (literal)

Abstract

• The haplophase of the pine blister rust Cronartium flaccidum was used to grow mycelial clones axenically from single genotypes. Single telia were suspended over the media in order to obtain a direct cast of uninucleate basidiospores, providing nurse cultures from a mass basidiospore inoculum. Nurse culture and medium composition were crucial factors in successful germination and establishment of single-spore colonies. Significant differences in percentage germination between treatments and controls suggested that an extracellular matrix secreted by high density seeded basidiospores could be involved in the germination of single propagules. Proteins present in this matrix could exert a surface contact stimulus triggering germination of newly seeded basidiospores. Pre-production of extracellular enzymes in the medium could also initiate early substrate catabolism and thus create a favourable environment to support subsequent colony establishment. Growth was good on various media, and excellent on HG1Y+BSA medium. Primary mother colonies were divided into several smaller ones, re-grown axenically, then macerated. Aliquots of the resulting mycelial fragments were dispensed with a micropipette into fresh agar media. Clones derived from each pure culture were in general morphologically similar in texture, margin, compactness, and surface topography. These genetically pure lines grew at a faster rate and rapidly produced a considerable amount of mycelium. The significance of culturing rust fungi from single genotypes is discussed in relation to potential applications and new directions of research. (literal)

Prodotto di

• Istituto per la protezione delle piante (IPP) (Istituto)

Autore CNR

• ALESSANDRO RAGAZZI (Unità di personale esterno)
• SALVATORE MORICCA (Unità di personale interno)

Insieme di parole chiave

• Keywords of "Establishment of single-genotype axenic cultures from the haploid stage of the pine blister rust Cronartium flaccidum." (Insieme di parole chiave)

Incoming links:

Autore CNR di

• SALVATORE MORICCA (Unità di personale interno)
• ALESSANDRO RAGAZZI (Unità di personale esterno)

Prodotto

• Istituto per la protezione delle piante (IPP) (Istituto)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Mycological research (Rivista)

Insieme di parole chiave di

• Keywords of "Establishment of single-genotype axenic cultures from the haploid stage of the pine blister rust Cronartium flaccidum." (Insieme di parole chiave)