Mode of action and antifungal properties of two cold-adapted chitinases. (Articolo in rivista)

Type
Label
  • Mode of action and antifungal properties of two cold-adapted chitinases. (Articolo in rivista) (literal)
Anno
  • 2003-01-01T00:00:00+01:00 (literal)
Alternative label
  • Mavromatis K., Lorito M., Woo S.L., Bouriotis V. (2003)
    Mode of action and antifungal properties of two cold-adapted chitinases.
    in Extremophiles (Tokyo, Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Mavromatis K., Lorito M., Woo S.L., Bouriotis V. (literal)
Pagina inizio
  • 385 (literal)
Pagina fine
  • 390 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 7 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • -Department of Biology, Division of Applied Biology and Biotechnology, University of Crete, Heraklion, Crete, Greece -Enzyme Technology Division, Institute of Molecular Biology and Biotechnology, Crete, Greece -Dipartmento di Arboricoltura, Botanica e Patologia Vegetale, Sezione Patologia Vegetale, Universita degli Studi di Napoli Federico II e Istituto per la Protezione delle Piante, CNR, Portici, Naples, Italy (literal)
Titolo
  • Mode of action and antifungal properties of two cold-adapted chitinases. (literal)
Abstract
  • The mode of action of two chitinases from the Antarctic Arthrobacter sp. strain TAD20 on N-acetyl-chitooligomers and chitin polymers has been elucidated. Identification of the length of chitin oligomers following enzymatic hydrolysis was verified by using HPLC-based analysis. It was observed that the length of the oligomer is important for enzyme action. The enzymes cannot effectively hydrolyze chitin oligomers with a degree of polymerization lower than four. ArChiA is an endochitinase which hydrolyzes chitin substrates randomly, whereas ArChiB is an exochitinase which degrades chitin chains and N-acetyl-chitooligomers from the nonreducing end, releasingN-N'-diacetyl-chitobiose. ArChiB (100 µg/ml) inhibited spore germination and hyphal elongation of the phytopathogenic fungus Botrytis cinerea by 15% and 30%, respectively. A more pronounced effect was observed with ArChiA (100 µg/ml) resulting in 70% inhibition of spore germination and 60% inhibition of germ tube elongation. A slight additive effect was observed, when the two enzymes were used in combination, only on the inhibition of germ tube elongation. (literal)
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