Inducible expression of a Phytolacca heterotepala ribosome-inactivating protein leads to enhanced resistance against major fungal pathogens in tobacco. (Articolo in rivista)

Type
Label
  • Inducible expression of a Phytolacca heterotepala ribosome-inactivating protein leads to enhanced resistance against major fungal pathogens in tobacco. (Articolo in rivista) (literal)
Anno
  • 2005-01-01T00:00:00+01:00 (literal)
Alternative label
  • Corrado G., Delli Bovi P., Ciliento R., Gaudio L., Di Maro A., Aceto S., Lorito M., Rao R. (2005)
    Inducible expression of a Phytolacca heterotepala ribosome-inactivating protein leads to enhanced resistance against major fungal pathogens in tobacco.
    in Phytopathology
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Corrado G., Delli Bovi P., Ciliento R., Gaudio L., Di Maro A., Aceto S., Lorito M., Rao R. (literal)
Pagina inizio
  • 206 (literal)
Pagina fine
  • 215 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 95 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • -Dipartimento di Scienze del Suolo, della Pianta e dell'Ambiente, Università di Napoli Federico II, ITALIE -Dipartimento di Genetica, Biologia Generale e Molecolare, Università di Napoli Federico II, ITALIE -Dipartimento di Arboricoltura, Botanica e Patologia Vegetale, Università di Napoli Federico II, ITALIE -Dipartimento di Scienze della Vita, Seconda Università degli Studi di Napoli, ITALIE -Istituto per la Protezione delle Piante, CNR, Portici (NA) (literal)
Titolo
  • Inducible expression of a Phytolacca heterotepala ribosome-inactivating protein leads to enhanced resistance against major fungal pathogens in tobacco. (literal)
Abstract
  • Plant genetic engineering has long been considered a valuable tool to fight fungal pathogens because it would limit the economically costly and environmentally undesirable chemical methods of disease control. Ribosome-inactivating proteins (RIPS) are potentially useful for plant defense considering their antiviral and antimicrobial activities but their use is limited by their cytotoxic activity. A new gene coding for an RIP isolated from leaves of Phytolacca heterotepala was expressed in tobacco under the control of the wound-inducible promoter of the bean polygalacturonase-inhibiting protein I gene to increase resistance against different fungal pathogens, because an individual RIP isolated from P. heterotepala showed direct antifungal toxicity. Phenotypically normal transgenic lines infected with Alternaria alternata and Botrytis cinerea showed a significant reduction of leaf damage while reverse transcription-polymerase chain reaction and western analysis indicated the expression of the RIP transgene upon wounding and pathogen attack. This work demonstrates that use of a wound-inducible promoter is useful to limit the accumulation of antimicrobial phytotoxic proteins only in infected areas and that the controlled expression of the PhRIP I gene can be very effective to control fungal pathogens with different phytopathogenic actions. (literal)
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