A non-phenol-chloroform extraction of double-stranded RNA from plant and fungal tissues. (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• A non-phenol-chloroform extraction of double-stranded RNA from plant and fungal tissues. (Articolo in rivista) (literal)

Anno

• 2008-01-01T00:00:00+01:00 (literal)

Alternative label

• Baljjia A., Kvarnheden A., Turchetti T. (2008)
  A non-phenol-chloroform extraction of double-stranded RNA from plant and fungal tissues.
  in Journal of virological methods
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Baljjia A., Kvarnheden A., Turchetti T. (literal)

Pagina inizio

• 32 (literal)

Pagina fine

• 37 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 152 (literal)

Rivista

• Journal of virological methods (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• - Baljjia A., Turchetti T., CNR, Istituto per la Protezione delle Piante, Sezione di Firenze, Via Madonna del Piano 10, 50019 Sesto Fiorentino (FI), Italia - Kvarnheden A., Department of Plant Biology and Forest Genetics, Uppsala BioCenter SLU, Box 7080, SE-750 07 Uppsala, Sweden (literal)

Titolo

• A non-phenol-chloroform extraction of double-stranded RNA from plant and fungal tissues. (literal)

Abstract

• Double-stranded RNA (dsRNA) molecules of viruses are found in nature at a very high frequency. Their detection in plants and fungi has been carried out with difficulty due to the complicated dsRNA extraction techniques used commonly which includes phenol-chloroform extractions. In this study, an extraction method for isolation of dsRNA is described that is free of phenol and chloroform. A lysis buffer, containing beta-mercaptoethanol and polyvinylpolypyrrolidone (PVPP-40), was added to homogenised tissues and the subsequent supernatant was filtered through a cellulose CF-11 mini-column. DsRNA molecules were separated based on the differing affinity of nucleic acids for the cellulose CF-11 resin in 20% ethanol buffer. This easy, rapid and cheap technique has been successfully tested on fungi and plants containing different dsRNA virus molecules, indicating the possibility of a wide use of the method. (literal)

Prodotto di

• Impatto economico e ambientale di fattori di stress su piante ed ecosistemi agroforestali produttivi. (AG.P04.003.001) (Modulo)
• Istituto per la protezione delle piante (IPP) (Istituto)

Autore CNR

• ALITUKIRIZA BALIJJA (Persona)
• TULLIO TURCHETTI (Unità di personale interno)

Insieme di parole chiave

• Parole chiave di "A non-phenol-chloroform extraction of double-stranded RNA from plant and fungal tissues." (Insieme di parole chiave)

Incoming links:

Prodotto

• Istituto per la protezione delle piante (IPP) (Istituto)
• Impatto economico e ambientale di fattori di stress su piante ed ecosistemi agroforestali produttivi. (AG.P04.003.001) (Modulo)

Autore CNR di

• TULLIO TURCHETTI (Unità di personale interno)
• ALITUKIRIZA BALIJJA (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of virological methods (Rivista)

Insieme di parole chiave di

• Parole chiave di "A non-phenol-chloroform extraction of double-stranded RNA from plant and fungal tissues." (Insieme di parole chiave)