http://www.cnr.it/ontology/cnr/individuo/prodotto/ID4101
An Archaeal endoribonuclease catalyzes cis- and trans- nonpliceosomal splicing in mouse cells. (Articolo in rivista)
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- An Archaeal endoribonuclease catalyzes cis- and trans- nonpliceosomal splicing in mouse cells. (Articolo in rivista) (literal)
- Anno
- 2003-01-01T00:00:00+01:00 (literal)
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- Deidda G., Rossi N. and Tocchini-Valentini G.P. (literal)
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- Commented in: Garcia-Blanco MA. Nat Biotechnol. 2003 21:1448-9 (literal)
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- Pubblicazione su rivista internazionale (literal)
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- CNR - Istituto di Biologia Cellulare (literal)
- Titolo
- An Archaeal endoribonuclease catalyzes cis- and trans- nonpliceosomal splicing in mouse cells. (literal)
- Abstract
- The tRNA endonuclease from the archaebacterium Methanococcus jannaschii (MJ endonuclease) can cleave RNAs forming
specific bulge-helix-bulge (BHB) structures recognized by the enzyme. The resulting cleavage products are subsequently
joined together by an endogenous ligase. We demonstrate the potential of using this strategy for repairing RNA in higher
organisms by expressing the enzyme in mouse cells. Reporter target mRNAs modified with 17-nucleotide introns, flanked
by sequences capable of forming BHB structures in cis, were expressed in mouse cells. RNA molecules that can form BHB
substrates in trans with targeted mRNAs were also designed. Co-transfection of mouse cells with plasmids expressing these
RNAs and the MJ endonuclease led to formation of RNA chimeras in which the target and exogenous RNA were
recombined across the BHB. This technology is not limited to mRNA, but could in principle be used to destroy, modify or
restore the function of a vast repertoire of RNA species or to join selectable tags to target RNAs. (literal)
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