Characterisation of RC-proteoliposomes at different RC/lipid ratios (Articolo in rivista)

Type
Label
  • Characterisation of RC-proteoliposomes at different RC/lipid ratios (Articolo in rivista) (literal)
Anno
  • 2009-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1007/s11120-009-9423-5 (literal)
Alternative label
  • Milano,F. Italiano, F.; Agostiano, A.; Trotta, M. (2009)
    Characterisation of RC-proteoliposomes at different RC/lipid ratios
    in Photosynthesis research (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Milano,F. Italiano, F.; Agostiano, A.; Trotta, M. (literal)
Pagina inizio
  • 107 (literal)
Pagina fine
  • 112 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 100 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • CNR, Istituto per i Processi Chimico-Fisici, Sezione di Bari Dipartimento di Chimica, Università degli studi di Bari (literal)
Titolo
  • Characterisation of RC-proteoliposomes at different RC/lipid ratios (literal)
Abstract
  • Reconstitution of membrane proteins in phospholipid vesicles allows the investigation of such macromolecules in a biomimetic simplified environment. The often employed micelle-to-vesicle-transition method for proteoliposome preparation is a fast and reproducible technique. In this, communication is shown that the lipid/protein ratio influences the size of the proteoliposomes and the actual protein reconstitution. The results indicate that for photosynthetic reaction centres, the best conditions for ligand-interaction experiments are achieved with a lipid/protein value of 1000:1, while for complete protein incorporation, the 2000:1 ratio should be chosen. (literal)
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