http://www.cnr.it/ontology/cnr/individuo/prodotto/ID40564
Characterisation of RC-proteoliposomes at different RC/lipid ratios (Articolo in rivista)
- Type
- Label
- Characterisation of RC-proteoliposomes at different RC/lipid ratios (Articolo in rivista) (literal)
- Anno
- 2009-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1007/s11120-009-9423-5 (literal)
- Alternative label
Milano,F. Italiano, F.; Agostiano, A.; Trotta, M. (2009)
Characterisation of RC-proteoliposomes at different RC/lipid ratios
in Photosynthesis research (Print)
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Milano,F. Italiano, F.; Agostiano, A.; Trotta, M. (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- CNR, Istituto per i Processi Chimico-Fisici, Sezione di Bari
Dipartimento di Chimica, Università degli studi di Bari (literal)
- Titolo
- Characterisation of RC-proteoliposomes at different RC/lipid ratios (literal)
- Abstract
- Reconstitution of membrane proteins in phospholipid vesicles allows the investigation of such macromolecules in a biomimetic simplified environment. The often employed micelle-to-vesicle-transition method for proteoliposome preparation is a fast and reproducible technique. In this, communication is shown that the lipid/protein ratio influences the size of the proteoliposomes and the actual protein reconstitution. The results indicate that for photosynthetic reaction centres, the best conditions for ligand-interaction experiments are achieved with a lipid/protein value of 1000:1, while for complete protein incorporation, the 2000:1 ratio should be chosen. (literal)
- Prodotto di
- Autore CNR
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