Nanoelectrode ensembles as recognition platform for electrochemical immunosensors (Articolo in rivista)

Type
Label
  • Nanoelectrode ensembles as recognition platform for electrochemical immunosensors (Articolo in rivista) (literal)
Anno
  • 2008-01-01T00:00:00+01:00 (literal)
Alternative label
  • Mucelli, SP; Zamuner, M; Tormen, M; Stanta, G; Ugo, P (2008)
    Nanoelectrode ensembles as recognition platform for electrochemical immunosensors
    in Biosensors & bioelectronics
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Mucelli, SP; Zamuner, M; Tormen, M; Stanta, G; Ugo, P (literal)
Pagina inizio
  • 1900 (literal)
Pagina fine
  • 1903 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 23 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • \"[Zamuner, M.; Ugo, P.] Univ Venice, Dept Phys Chem, I-30123 Venice, Italy; [Mucelli, S. Pozzi; Stanta, G.] Int Ctr Genet Engn & Biotechnol, Mol Histopathol Lab, I-34012 Trieste, Italy; [Tormen, M.] CNR, INFM, Lab Nazl TASC, I-34012 Trieste, Italy; [Mucelli, S. Pozzi] European Ctr Sustainable Impact Nanotechnol, I-45100 Rovigo, Italy (literal)
Titolo
  • Nanoelectrode ensembles as recognition platform for electrochemical immunosensors (literal)
Abstract
  • \"in this study we demonstrate the possibility to prepare highly sensitive nanostructured electrochemical immunosensors by immobilizing biorecognition elements on nanoelectrode ensembles (NEEs) prepared in track-etch polycarbonate membranes. The gold nanodisk electrodes act as electrochemical transducers while the surrounding polycarbonate binds the antibody-based biorecognition layer. The interaction between target protein and antibody is detected by suitable secondary antibodies labelled with a redox enzyme. A redox mediator, added to the sample solution, shuttles electrons from the nanoelectrodes to the biorecognition layer, so generating an electrocatalytic signal. This allows one to fully exploit the highly improved signal-to-background current ratio, typical of NEEs. In particular, the receptor protein HER2 was studied as the target analyte. HER2 detection allows the identification of breast cancer that can be treated with the monoclonal antibody trastuzumab. NEEs were functionalized with trastuzumab which interacts specifically with HER2. The biorecognition process was completed by adding a primary antibody and a secondary antibody labelled with horseradish peroxidase. Hydrogen peroxide was added to modulate the label electroactivity; methylene blue was the redox mediator generating voltammetric signals. NEEs functionalized with trastuzumab were tested to detect small amounts of HER2 in diluted cell lysates and tumour lysates. (c) 2008 Elsevier B.V. All rights reserved.\" (literal)
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