http://www.cnr.it/ontology/cnr/individuo/prodotto/ID39918
Structure and Dynamics of High Molecular Weight Glutenin Subunits of Durum Wheat (Triticum durum) in Water and Alcohol Solutions Studied by Electron Paramagnetic Resonance and Circular Dichroism Spectroscopies (Articolo in rivista)
- Type
- Label
- Structure and Dynamics of High Molecular Weight Glutenin Subunits of Durum Wheat (Triticum durum) in Water and Alcohol Solutions Studied by Electron Paramagnetic Resonance and Circular Dichroism Spectroscopies (Articolo in rivista) (literal)
- Anno
- 2001-01-01T00:00:00+01:00 (literal)
- Alternative label
L. Calucci, C. Pinzino, A. Capocchi, L. Galleschi, S. Ghiringhelli, F. Saviozzi, M. Zandomeneghi (2001)
Structure and Dynamics of High Molecular Weight Glutenin Subunits of Durum Wheat (Triticum durum) in Water and Alcohol Solutions Studied by Electron Paramagnetic Resonance and Circular Dichroism Spectroscopies
in Journal of agricultural and food chemistry
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- L. Calucci, C. Pinzino, A. Capocchi, L. Galleschi, S. Ghiringhelli, F. Saviozzi, M. Zandomeneghi (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Istituto di Chimica Quantistica ed Energetica Molecolare del CNR, Area della Ricerca di Pisa, via V. Alfieri 1, I-56010 San Giuliano Terme, Pisa, Italy
Dipartimento di Scienze Botaniche, Universita` di Pisa, via L. Ghini 5, I-56126 Pisa, Italy
Dipartimento di Chimica e Chimica Industriale, Universita` di Pisa, via Risorgimento 35, I-56126 Pisa, Italy (literal)
- Titolo
- Structure and Dynamics of High Molecular Weight Glutenin Subunits of Durum Wheat (Triticum durum) in Water and Alcohol Solutions Studied by Electron Paramagnetic Resonance and Circular Dichroism Spectroscopies (literal)
- Abstract
- Complementary information on the structure and dynamics of high molecular weight glutenin
subunits (HMW-GS) of durum wheat (Triticum durum) was obtained by means of two spectroscopic
techniques. Electron paramagnetic resonance was used to investigate the dynamics of the HMWGS
hydrated with two 2-propanol/water mixtures at temperatures between 268 and 308 K by specific
spin labeling of their cysteine residues. Spectra were of a composite type, resulting from two
populations of spin labels differing in molecular mobility, both undergoing isotropic rotational
diffusion. Diffusional coefficients and populations of the fast- and slow-moving spin labels, determined
by an accurate spectral line shape analysis, are discussed as a function of temperature and water
content in the solvent systems. Far-UV circular dichroism was employed to provide information on
the secondary structure of the HMW-GS in three different solvents [aqueous 50% (v/v) 2-propanol,
aqueous 0.1% (v/v) trifluoroacetic acid, and trifluoroethanol]. For the first one, the influence of
temperature on HMW-GS structure was also investigated. (literal)
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