Structure and Dynamics of High Molecular Weight Glutenin Subunits of Durum Wheat (Triticum durum) in Water and Alcohol Solutions Studied by Electron Paramagnetic Resonance and Circular Dichroism Spectroscopies (Articolo in rivista)

Type
Label
  • Structure and Dynamics of High Molecular Weight Glutenin Subunits of Durum Wheat (Triticum durum) in Water and Alcohol Solutions Studied by Electron Paramagnetic Resonance and Circular Dichroism Spectroscopies (Articolo in rivista) (literal)
Anno
  • 2001-01-01T00:00:00+01:00 (literal)
Alternative label
  • L. Calucci, C. Pinzino, A. Capocchi, L. Galleschi, S. Ghiringhelli, F. Saviozzi, M. Zandomeneghi (2001)
    Structure and Dynamics of High Molecular Weight Glutenin Subunits of Durum Wheat (Triticum durum) in Water and Alcohol Solutions Studied by Electron Paramagnetic Resonance and Circular Dichroism Spectroscopies
    in Journal of agricultural and food chemistry
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • L. Calucci, C. Pinzino, A. Capocchi, L. Galleschi, S. Ghiringhelli, F. Saviozzi, M. Zandomeneghi (literal)
Pagina inizio
  • 359 (literal)
Pagina fine
  • 365 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 49 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Istituto di Chimica Quantistica ed Energetica Molecolare del CNR, Area della Ricerca di Pisa, via V. Alfieri 1, I-56010 San Giuliano Terme, Pisa, Italy Dipartimento di Scienze Botaniche, Universita` di Pisa, via L. Ghini 5, I-56126 Pisa, Italy Dipartimento di Chimica e Chimica Industriale, Universita` di Pisa, via Risorgimento 35, I-56126 Pisa, Italy (literal)
Titolo
  • Structure and Dynamics of High Molecular Weight Glutenin Subunits of Durum Wheat (Triticum durum) in Water and Alcohol Solutions Studied by Electron Paramagnetic Resonance and Circular Dichroism Spectroscopies (literal)
Abstract
  • Complementary information on the structure and dynamics of high molecular weight glutenin subunits (HMW-GS) of durum wheat (Triticum durum) was obtained by means of two spectroscopic techniques. Electron paramagnetic resonance was used to investigate the dynamics of the HMWGS hydrated with two 2-propanol/water mixtures at temperatures between 268 and 308 K by specific spin labeling of their cysteine residues. Spectra were of a composite type, resulting from two populations of spin labels differing in molecular mobility, both undergoing isotropic rotational diffusion. Diffusional coefficients and populations of the fast- and slow-moving spin labels, determined by an accurate spectral line shape analysis, are discussed as a function of temperature and water content in the solvent systems. Far-UV circular dichroism was employed to provide information on the secondary structure of the HMW-GS in three different solvents [aqueous 50% (v/v) 2-propanol, aqueous 0.1% (v/v) trifluoroacetic acid, and trifluoroethanol]. For the first one, the influence of temperature on HMW-GS structure was also investigated. (literal)
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