Evidence for Anterograde Transport and Transcytosis of Botulinum Neurotoxin A (BoNT/A) (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Evidence for Anterograde Transport and Transcytosis of Botulinum Neurotoxin A (BoNT/A) (Articolo in rivista) (literal)

Anno

• 2011-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1523/JNEUROSCI.2618-11.2011 (literal)

Alternative label

• Restani, Laura; Antonucci, Flavia; Gianfranceschi, Laura; Rossi, Chiara; Rossetto, Ornella; Caleo, Matteo (2011)
  Evidence for Anterograde Transport and Transcytosis of Botulinum Neurotoxin A (BoNT/A)
  in The Journal of neuroscience
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Restani, Laura; Antonucci, Flavia; Gianfranceschi, Laura; Rossi, Chiara; Rossetto, Ornella; Caleo, Matteo (literal)

Pagina inizio

• 15650 (literal)

Pagina fine

• 15659 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 31 (literal)

Rivista

• ?The ?Journal of neuroscience (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 10 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 44 (literal)

Note

• ISI Web of Science (WOS) (literal)
• ISI Web of Science (WoS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Consiglio Nazionale delle Ricerche (CNR); University of Padua (literal)

Titolo

• Evidence for Anterograde Transport and Transcytosis of Botulinum Neurotoxin A (BoNT/A) (literal)

Abstract

• Botulinum neurotoxin type A (BoNT/A) is a metalloprotease that blocks synaptic transmission via the cleavage of SNAP-25 (synaptosomal-associated protein of 25 kDa). BoNT/A is successfully used in clinical neurology for the treatment of several neuromuscular pathologies and pain syndromes. Despite its widespread use, relatively little is known on BoNT/A intracellular trafficking in neurons. Using the visual pathway as a model system, here we show that catalytically active BoNT/A is capable of undergoing anterograde axonal transport and transcytosis. Following BoNT/A injection into the rat eye, significant levels of BoNT/A-cleaved SNAP-25 appeared in the retinorecipient layers of the superior colliculus (SC). Anterograde propagation of BoNT/A effects required axonal transport, ruling out a systemic spread of the toxin. Cleaved SNAP-25 was present in presynaptic structures of the tectum, but retinal terminals were devoid of the immunoreactivity, indicative of transcytosis. Experiments based on sequential administration of BoNT/A and BoNT/E showed a persistent catalytic activity of BoNT/A in tectal cells following its injection into the retina. Our findings demonstrate that catalytically active BoNT/A is anterogradely transported from the eye to the SC and transcytosed to tectal synapses. These data are important for a more complete understanding of the mechanisms of action of BoNT/A. (literal)

Prodotto di

• Istituto di neuroscienze (IN) (Istituto)
• Plasticità e invecchiamento del sistema nervoso (ME.P02.006.001) (Modulo)

Autore CNR

• FLAVIA ANTONUCCI (Unità di personale esterno)
• LAURA RESTANI (Unità di personale esterno)
• MATTEO CALEO (Unità di personale interno)
• CHIARA ROSSI (Persona)
• LAURA GIANFRANCESCHI (Persona)

Incoming links:

Autore CNR di

• MATTEO CALEO (Unità di personale interno)
• LAURA GIANFRANCESCHI (Persona)
• FLAVIA ANTONUCCI (Unità di personale esterno)
• LAURA RESTANI (Unità di personale esterno)
• CHIARA ROSSI (Persona)

Prodotto

• Istituto di neuroscienze (IN) (Istituto)
• Plasticità e invecchiamento del sistema nervoso (ME.P02.006.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• ?The ?Journal of neuroscience (Rivista)