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Calcitonin gene-related peptide (CGRP) triggers Ca2+ responses in cultured astrocytes and in Bergmann glial cells from cerebellar slices (Articolo in rivista)

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Calcitonin gene-related peptide (CGRP) triggers Ca2+ responses in cultured astrocytes and in Bergmann glial cells from cerebellar slices (Articolo in rivista) (literal)

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Morara S; Wang LP; Filippov V; Dickerson IM; Grohovaz F; Provini L; Kettenmann H (2008)
Calcitonin gene-related peptide (CGRP) triggers Ca2+ responses in cultured astrocytes and in Bergmann glial cells from cerebellar slices
in European journal of neuroscience (Print); Oxford University Press, Oxford (Regno Unito)
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Morara S; Wang LP; Filippov V; Dickerson IM; Grohovaz F; Provini L; Kettenmann H (literal)

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CORRIGENDUM: European Journal of Neuroscience, Vol. 28, p. 2550, 2008 doi:10.1111/j.1460-9568.2008.06554 In the published paper of Morara et al. (2008) there was an error in the penultimate sentence of the Fig. 2 legend. It should have read ''Note the delayed persistent increase in calcium activity that followed the second application of CGRP.'' We apologise for any inconvenience caused. (literal)

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Stefano Morara,1; Li-Ping Wang,2; Vitaly Filippov,2; Ian M. Dickerson,3; Fabio Grohovaz,4,5,6; Luciano Provini,1,7; Helmut Kettenmann,2 1 C. N. R., Institute of Neuroscience, Via Privata Bianco 9, 20131 Milan, Italy 2 Max Delbru¨ck Center for Molecular Medicine, Berlin, Germany 3 Department of Neurobiology, University of Rochester, Rochester, NY, USA 4 San Raffaele Scientific Institute, San Raffaele University, Milan, Italy 5 Research Unit of Molecular Neuroscience, Italian Institute of Technology, Milan, Italy 6 National Institute of Neuroscience, Milan, Italy 7 Institute of General Physiology and Chemical Biology, Universita` degli Studi, Milan, Italy (literal)

Titolo

Calcitonin gene-related peptide (CGRP) triggers Ca2+ responses in cultured astrocytes and in Bergmann glial cells from cerebellar slices (literal)

Abstract

The neuropeptide calcitonin gene-related peptide (CGRP) is transiently expressed in cerebellar climbing fibers during development while its receptor is mainly expressed in astrocytes, in particular Bergmann glial cells. Here, we analyzed the effects of CGRP on astrocytic calcium signaling. Mouse cultured astrocytes from cerebellar or cerebral cortex as well as Bergmann glial cells from acutely isolated cerebellar slices were loaded with the Ca2+ sensor Fura-2. CGRP triggered transient increases in intracellular Ca2+ in astrocytes in culture as well as in acute slices. Responses were observed in the concentration range of 1 nm to 1 mm, in both the cell body and its processes. The calcium transients were dependent on release from intracellular stores as they were blocked by thapsigargin but not by the absence of extracellular calcium. In addition, after CGRP application a further delayed transient increase in calcium activity could be observed. Finally, cerebellar astrocytes from neonatal mice expressed receptor component protein, a component of the CGRP receptor, as revealed by immunofluorescence and confocal microscopy. It is thus proposed that the CGRPcontaining afferent fibers in the cerebellum (the climbing fibers) modulate calcium in astrocytes by releasing the neuropeptide during development and hence possibly influence the differentiation of Purkinje cells. (literal)

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