Endogenous ƒgamma-aminobutyric acid (GABA)A receptor active neurosteroids and the sedative/hypnotic action of ƒgamma-hydroxybutyric acid (GHB): A study in GHB-S (sensitive) and GHB-R (resistant) rat lines (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Endogenous ƒgamma-aminobutyric acid (GABA)A receptor active neurosteroids and the sedative/hypnotic action of ƒgamma-hydroxybutyric acid (GHB): A study in GHB-S (sensitive) and GHB-R (resistant) rat lines (Articolo in rivista) (literal)

Anno

• 2005-01-01T00:00:00+01:00 (literal)

Alternative label

• Barbaccia M.L., Carai M.A.M., Colombo G., Lobina C., Purdy R.H., Gessa G.L. (2005)
  Endogenous ƒgamma-aminobutyric acid (GABA)A receptor active neurosteroids and the sedative/hypnotic action of ƒgamma-hydroxybutyric acid (GHB): A study in GHB-S (sensitive) and GHB-R (resistant) rat lines
  in Neuropharmacology
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Barbaccia M.L., Carai M.A.M., Colombo G., Lobina C., Purdy R.H., Gessa G.L. (literal)

Pagina inizio

• 48 (literal)

Pagina fine

• 58 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 49 (literal)

Rivista

• Neuropharmacology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 11 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 1 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Department of Neuroscience, University of Rome \"Tor Vergata\", Rome, Italy; Department of Neuroscience, University of Cagliari, Cagliari, Italy; CNR Institute of Neuroscience, Section of Cagliari, Cagliari, Italy; The Scripps Research Institute, La Jolla, CA, USA. (literal)

Titolo

• Endogenous ƒgamma-aminobutyric acid (GABA)A receptor active neurosteroids and the sedative/hypnotic action of ƒgamma-hydroxybutyric acid (GHB): A study in GHB-S (sensitive) and GHB-R (resistant) rat lines (literal)

Abstract

• In rat brain, gamma-hydroxybutyric-acid (GHB) increases the concentrations of 3alpha-hydroxy,5alpha-pregnan-20-one (allopregnanolone, 3alpha,5alpha-THP) and 3alpha,21-dihydroxy,5alpha-pregnan-20-one (allotetrahydrodeoxycorticosterone/3alpha,5alphaTHDOC), two neurosteroids acting as positive allosteric modulators of gamma-aminobutyric acid (GABA)A receptors. This study was aimed at assessing whether neurosteroids play a role in GHB-induced loss of righting reflex (LORR). Basal and GHB-stimulated brain concentrations of endogenous 3alpha,5alpha-THP and 3alpha,5alpha-THDOC were analyzed in two rat lines, GHB-sensitive (GHB-S) and GHB-resistant (GHB-R), selectively bred for opposite sensitivity to GHB-induced sedation/hypnosis. Basal neurosteroid concentrations were similar in brain cortex of the two rat lines. However, GHB (1000 mg/kg, i.p., 30 minutes) increased the brain cortical concentrations of 3alpha,5alpha-THP and 3alpha,5alpha-THDOC 7 and 2.5 fold, respectively, in male GHB-S and only 4 and 2 fold, respectively, in male GHB-R rats. In GHB-S rats this increase lasted up to 90 minutes and declined 180 minutes following GHB administration, a time course that matches LORR onset and duration. In contrast, in GHB-R rats, which failed to show GHB-induced LORR, brain cortical 3alpha,5alpha-THP and 3alpha,5alpha-THDOC were back to control values by 90 minutes. Brain cortical 3alpha,5alpha-THP and 3alpha,5alpha-THDOC at LORR onset were increased to the same extent (2-3 fold) as in GHB-S females in those few female GHB-R rats that lost the righting reflex after GHB administration, but not in female GHB-R rats that failed to show LORR. Sub-hypnotic doses (7.5 and 12.5 mg/kg, i.p.) of pregnanolone, administered 10 minutes before GHB, dose-dependently facilitated the expression of GHB-induced LORR in GHB-R male rats. These results suggest that the GHB-induced increases of brain 3alpha,5alpha-THP and 3alpha,5alpha-THDOC concentrations have a permissive action in GHB's sedative/hypnotic action. (literal)

Prodotto di

• Istituto di neuroscienze (IN) (Istituto)
• Neurobiologia dell'alcolismo (ME.P02.020.001) (Modulo)

Autore CNR

• GIAN LUIGI GESSA (Unità di personale esterno)
• GIANCARLO COLOMBO (Unità di personale interno)

Insieme di parole chiave

• Parole chiave di "Endogenous ƒgamma-aminobutyric acid (GABA)A receptor active neurosteroids and the sedative/hypnotic action of ƒgamma-hydroxybutyric acid (GHB): A study in GHB-S (sensitive) and GHB-R (resistant) rat lines" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• GIANCARLO COLOMBO (Unità di personale interno)
• GIAN LUIGI GESSA (Unità di personale esterno)

Prodotto

• Istituto di neuroscienze (IN) (Istituto)
• Neurobiologia dell'alcolismo (ME.P02.020.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Neuropharmacology (Rivista)

Insieme di parole chiave di

• Parole chiave di "Endogenous ƒgamma-aminobutyric acid (GABA)A receptor active neurosteroids and the sedative/hypnotic action of ƒgamma-hydroxybutyric acid (GHB): A study in GHB-S (sensitive) and GHB-R (resistant) rat lines" (Insieme di parole chiave)