Biocompatible surfactants for water-in-fluorocarbon emulsions (Articolo in rivista)

Type
Label
  • Biocompatible surfactants for water-in-fluorocarbon emulsions (Articolo in rivista) (literal)
Anno
  • 2008-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1039/b806706f (literal)
Alternative label
  • Holtze, C; Rowat, AC; Agresti, JJ; Hutchison, JB; Angile, FE; Schmitz, CHJ; Koster, S; Duan, H; Humphry, KJ; Scanga, RA; Johnson, JS; Pisignano, D; Weitz, DA; (2008)
    Biocompatible surfactants for water-in-fluorocarbon emulsions
    in Lab on a chip (Print); The Royal Society of Chemistry, Cambridge (Regno Unito)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Holtze, C; Rowat, AC; Agresti, JJ; Hutchison, JB; Angile, FE; Schmitz, CHJ; Koster, S; Duan, H; Humphry, KJ; Scanga, RA; Johnson, JS; Pisignano, D; Weitz, DA; (literal)
Pagina inizio
  • 1632 (literal)
Pagina fine
  • 1639 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 8 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 8 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • \"[Holtze, C.; Rowat, A. C.; Agresti, J. J.; Angile, F. E.; Schmitz, C. H. J.; Koster, S.; Duan, H.; Humphry, K. J.; Weitz, D. A.] Harvard Univ, Sch Engn & Appl Sci, Cambridge, MA 02138 USA; [Hutchison, J. B.; Scanga, R. A.; Johnson, J. S.] Raindance Technol, Bedford, MA USA; [Angile, F. E.; Pisignano, D.] Univ Salento, INFM, CNR, Natl Nanotechnol Lab, Lecce, Italy; [Holtze, C.; Rowat, A. C.; Agresti, J. J.; Angile, F. E.; Schmitz, C. H. J.; Koster, S.; Duan, H.; Humphry, K. J.; Weitz, D. A.] Harvard Univ, Dept Phys, Cambridge, MA 02138 USA (literal)
Titolo
  • Biocompatible surfactants for water-in-fluorocarbon emulsions (literal)
Abstract
  • Drops of water-in-fluorocarbon emulsions have great potential for compartmentalizing both in vitro and in vivo biological systems; however, surfactants to stabilize such emulsions are scarce. Here we present a novel class of fluorosurfactants that we synthesize by coupling oligomeric perfluorinated polyethers (PFPE) with polyethyleneglycol (PEG). We demonstrate that these block copolymer surfactants stabilize water-in-fluorocarbon oil emulsions during all necessary steps of a drop-based experiment including drop formation, incubation, and reinjection into a second microfluidic device. Furthermore, we show that aqueous drops stabilized with these surfactants can be used for in vitro translation (IVT), as well as encapsulation and incubation of single cells. The compatability of this emulsion system with both biological systems and polydimethylsiloxane (PDMS) microfluidic devices makes these surfactants ideal for a broad range of high-throughput, drop-based applications. (literal)
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